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Highest antigen sequence indentity to the following orthologs: Mouse (59%), Rat (59%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-61148. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Juvenile nephronophthisis type 1 is caused by mutations of NPHP1, the gene encoding for nephrocystin. Nephrocystin interacts with p130Cas (BCAR1), proline-rich tyrosine kinase-2 (PTK2B or Pyk2), and tensin in embryonic kidney and testis, indicating that these proteins participate in a common signaling pathway. Nephrocystin and p130Cas interact in mammalian cells and both proteins prominently localize at or near sites of cell-cell contact in polarized Madin-Darby canine kidney epithelial cells. Expression of nephrocystin results in phosphorylation of Pyk2 on Tyrosine 402 as well as activation of downstream mitogen-activated protein kinases, such as ERK1 and ERK2. Nephrocystin contains a src-homology 3 (SH3) domain, which is highly conserved throughout evolution.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: Juvenile nephronophthisis 1 protein; Nephrocystin-1; Nephrocystin1; nephronophthisis 1 (juvenile)
基因别名: JBTS4; NPH1; NPHP1; SLSN1
UniProt ID: (Human) O15259
Entrez Gene ID: (Human) 4867