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产品信息
RP-070
应用
建议稀释比
已发表文章
产品规格
种属
Human
已发表种属
Human
Expression System
E. coli
分子量
46.7 kDa
分类
Recombinant
类型
Protein
纯度
>80%
活性
For furin inhibition assays, cells should be treated with 8.0 - 20 µM human alpha-1 PDX recombinant protein for 1 hour. For in vitro assays, 0.1 - 2.0 µM human alpha-1 PDX recombinant protein should be used.
偶联物
形式
Lyophilized
浓度
2.5 mg/mL
纯化类型
purified
保存液
PBS, pH 7.2
内含物
no preservative
保存条件
4° C, do not freeze
运输条件
Wet ice
产品详细信息
RP-070 was obtained from E. coli transformed with the alpha1-PDX gene. RP-070 can be used in furin inhibition assays. For furin inhibition assays, cells should be treated with 8.0-20 µM alpha1-PDX for 1 hour (see above references). For in vitro assays, 0.1-2.0 µM alpha1-PDX should be used. Purity: >80% (most lots are 80-95%) NOTE: Freezing this product may result in damage of the protein. NOTE: Once rehydrated, this protein retains optimal reactivity for 2-4 days at 4 degrees C. Longer storage time can be achieved by storing in a serum rich medium. RP-070 can be used for protein biochemical, intracellular trafficking, and protein kinetic assays.
Molecular weight is approximately 46.7 kDa.
靶标信息
Alpha1-PDX is a potent and selective inhibitor of furin (Ki = 0.6 nM). Alpha1-PDX was engineered by the construction of a variant of alpha1-antitrypsin that contains a minimal furin consensus sequence, RXXR. Cellular expression of alpha1-PDX effectively blocks the furin-dependent processing of protein precursors. In addition, extracellular application of alpha1-PDX can also block the processing of furin substrates. Alpha1-PDX has been shown to inhibit the processing of biologically significant proteins including many viral envelope glycoproteins, bacterial toxins, receptors and growth factors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: alpha1 PDX; Pyruvate Dehydrogenase Complex Component-X
