Invitrogen

Rat IFN-beta Recombinant Protein

产品信息

134001

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

产品规格

种属

Mouse, Rat

Expression System

CHO cells

分子量

19.68 kDa

分类

Recombinant

类型

Protein

纯度

>95%

内毒素浓度

<1 EU/µg

活性

EC50 ~ 1 U/mL; determined by use of the cytopathic effect inhibition assay described in [Rubinstein, S. et al. (1981). J. Virol. 37, 755-758]; [Familletti, P.C. et al. (1981). Methods in Enzymology, Vol. 78, 387-394]As there is no international Rat IFN-alpha standard, units are determined with respect to the international reference standard for mouse interferon alpha/beta (mouse IFN-alpha/beta) provided by the National Institutes of Health.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

纯化类型

purified

保存液

PBS with 0.125M trehalose

内含物

no preservative

保存条件

4° C

运输条件

Wet ice

产品详细信息

After receipt, this product should be kept at -20ºC while lyophilized and at -70°C after reconstitution in sterile distilled water for retention of full activity. After reconstitution, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. After thawing, this product is stable for at least four weeks at 4ºC. Further dilute only in buffers containing protein such as 0.1% bovine serum albumin (BSA).

靶标信息

Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by Natural IFN-producing cells (IPCs) as part of the host immune response. IFNs can also inhibit the development of tumors. IFN-beta binding results in the activation of the tyrosine kinases Jak1 and Tyk2, phosphorylation of members of the STAT family of transcription factors, and the transcription and expression of the immune response genes. More recently, several members of the toll-like receptor (TLR) family were found to stimulate the production IFN-beta. IFN-beta is currently used clinically for treatment of tumors, infections and multiple sclerosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。