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产品信息
RRIL18I
应用
建议稀释比
已发表文章
产品规格
种属
Rat
Expression System
P. pastoris
氨基酸序列
Rat IL-18, amino acids 37-194 (Accession # P97636)
分子量
18.3 kDa
分类
Recombinant
类型
Protein
纯度
>95% by SDS-PAGE
活性
ED50 = 20 - 40 ng/mL; determined by the dose-dependent induction of IFN-gamma production from KG-1 cells in the presence or recombinant mouse IL-12.
偶联物
形式
Lyophilized
纯化类型
purified
保存液
PBS
内含物
no preservative
保存条件
4° C
运输条件
Wet ice
产品详细信息
Mature rat IL-18 was expressed in Pichia pastoris, X-33. GenBank® Accession # P97636, amino acids 37 - 194. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: HFGRLHCTTA. Purity is >95% by SDS-PAGE analyses.
Activity: The biological activity of recombinant rat IL-18 was determined by its ability to induce IFNg production from KG-1 cells in the presence of recombinant mouse IL-12. 480 pg/mL and 610 pg/mL of IFNg were induced by 20 ng/mL and 40 ng/mL of recombinant rat IL-18, respectively.
Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.
Reconstitution and Dilution: Store lyophilized material at 4ºC. Reconstitute in 0.5 mL of distilled or deionized water. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70ºC, avoiding multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.
靶标信息
The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: IFN-gamma-inducing factor; il 18; IL-1 gamma; IL-18; ILN; Interferon gamma-inducing factor; interferon-gamma-inducing factor; Interleukin; Interleukin-1 gamma; Interleukin-18; Interleukin18; MGC12320
基因别名: Igif; IL-18; Il18
UniProt ID: (Rat) P97636
Entrez Gene ID: (Rat) 29197
