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产品信息
RRIL2I
应用
建议稀释比
已发表文章
产品规格
种属
Rat
Expression System
P. pastoris
氨基酸序列
Rat IL-2, amino acids 21-155 (Accession # P04351)
分子量
15.5 kDa
分类
Recombinant
类型
Protein
纯度
>95% by SDS-PAGE
活性
ED50 = 3 ng/mL; determined by the dose-dependent proliferation of mouse CTLL-2 cells.
偶联物
形式
Lyophilized
纯化类型
purified
保存液
PBS
内含物
no preservative
保存条件
4° C
运输条件
Wet ice
产品详细信息
Mature rat IL-2 was expressed in Pichia pastoris, X-33. GenBank Accession # P04351, amino acids 21 - 155. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: APTSSPAKET. Purity is >95% by SDS-PAGE analyses.
Activity: The biological activity of recombinant rat IL-2 was determined by measuring the proliferation of mouse CTLL-2 cells. The ED50 (or 1 unit of activity) for this effect is typically 3 ng/mL.
Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.
Reconstitution and Dilution: Store lyophilized material at 4ºC. Reconstitute in 0.5 mL of distilled or deionized water. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70ºC, avoiding multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.
靶标信息
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; M-IL-2; T-cell growth factor; T-cell growth factor (TCGF); TCGF
基因别名: Il-2; Il2
UniProt ID: (Rat) P17108
Entrez Gene ID: (Rat) 116562
