Search Thermo Fisher Scientific
![Human AKT (Phospho) [pS473] ELISA Kit](https://qa1.thermofisher.cn/antibody/images/650/KHO0111_Bar_080130.jpg?time=20240412 "Human AKT (Phospho) [pS473] ELISA Kit")
图: 1 / 7
Human AKT (Phospho) [pS473] ELISA Kit
Akt [pS473] phosphoELISA™. Jurkat cells were treated with wortmannin at increasing concentrations, lysed and assayed in parallel for Akt [Total] and Akt [pS473]. The level of total Akt remained comparable, while the levels of phosphorylation at serine residue 473 decreased with increasing dose of wortmannin.
![Human AKT (Phospho) [pS473] ELISA Kit](https://qa1.thermofisher.cn/antibody/images/150/KHO0111_Bar_080130.jpg?time=20240412)
![Human AKT (Phospho) [pS473] ELISA Kit](https://qa1.thermofisher.cn/antibody/images/150/KHO0111_Bar_071109.jpg?time=20240412)
![Human AKT (Phospho) [pS473] ELISA Kit](https://qa1.thermofisher.cn/antibody/images/150/KHO0111_SC_080321.jpg?time=20240412)
![Human AKT (Phospho) [pS473] ELISA Kit](https://qa1.thermofisher.cn/antibody/images/150/KHO0111_PC_080130.jpg?time=20240412)
![Human AKT (Phospho) [pS473] ELISA Kit](https://qa1.thermofisher.cn/antibody/images/150/4077.jpg?time=20240412)
![Human AKT (Phospho) [pS473] ELISA Kit (KHO0111)](https://qa1.thermofisher.cn/antibody/images/150/Sandwich-Direct-ELISA-Kit-Packaging-650x600.jpg?time=20240412)
►
产品规格
检测灵敏度
<0.8 U/mL
测定范围
1.6-100 U/mL
检测样本类型/体积
{Cell Lysate=10 µL}
手工操作时间
1 hr 20 min
总时间
4 hr
均相（无需洗涤）
No
批间差异
8.3%
批内差异
6.9%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
AKT1
Target site-specificity
Phosphorylated Ser473
蛋白亚型
Serine/Threonine Kinases
蛋白家族
Kinases & Inhibitors
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human AKT (Phospho) [pS473] ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of AKT (Phospho) [pS473] in fresh or frozen human cell lysates. The assay recognizes both natural and recombinant AKT (Phospho) [pS473].
原理方法
A monoclonal capture antibody specific for AKT (Phospho) [pS473] has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : AKT, AKT1, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
基因ID : (Human) 207
基因符号 : AKT1
蛋白别名 : AKT1m, CAKT, C-AKT, PKB beta, PKB gamma, PKBG, RAC-PK-beta, RAC-PK-gamma, PKB, PKB alpha, Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, rac protein kinase alpha, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha, serine-threonine protein kinase, v-akt murine thymoma viral oncogene homolog 1, v-akt murine thymoma viral oncogene-like protein 1
UniProt ID (Human) P31749
