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产品规格
检测灵敏度
2.5 pg/mL
测定范围
2.44-10,000 pg/mL
检测样本类型/体积
{Plasma=2 µL, Serum=2 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
ApoC3
蛋白家族
GPCR (G-Protein Coupled Receptors)
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Apolipoprotein C3 (Hu ApoC3) ELISA quantitates Hu ApoC3 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ApoC3.
原理方法
The Human ApoC3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Apolipoprotein C-III, also known as apo-CIII, is a protein that in humans is encoded by the APOC3 gene. Apo-CIII is a component of very low density lipoprotein (VLDL). APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to delay catabolism of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : APOC3, APOCIII, HALP2
基因ID : (Human) 345
基因符号 : APOC3
蛋白别名 : Apo-CIII, ApoC-III, Apolipoprotein C3, MGC150353, Apolipoprotein, apoC-III, Apolipoprotein C-III
UniProt ID (Human) P02656
