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产品规格
检测灵敏度
9.1 pg/mL
测定范围
18,8-1200
检测样本类型/体积
{Serum=50 µL, Supernatant=50 µL, Plasma=50 µL}
总时间
3 hr 10 min
均相（无需洗涤）
No
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Coated Microwell Strips
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
运输条件
Wet ice
储存
2-8°C
蛋白名称
IL-2
蛋白家族
Hormones & Receptors
均相（无需洗涤）
Human
Species (reported)
Human
检测试剂盒格式
Basic ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human Interleukin-2 (Hu IL-2) ELISA kit facilitates the accurate quantification of Hu IL-2 in various biological samples, including human serum, plasma, or cell culture medium. This highly specific assay is designed to exclusively detect both endogenous and recombinant forms of Hu IL-2.
原理方法
The Human IL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) employs a meticulously paired set of antibodies to capture and measure the target analyte. The microplate wells are pre-coated with a target-specific antibody that allows for the binding of the analyte during the assay. Upon the addition of samples or standards to the wells, they specifically bind to the immobilized (capture) antibody. The formation of a sandwich complex is achieved by introducing a second (detector) antibody. Subsequently, a substrate solution is added, initiating a reaction with the enzyme-antibody-target complex and generating a measurable signal. The intensity of this signal is directly proportional to the concentration of the target present in the original specimen.
靶标信息
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : IL-2, IL2, lymphokine, TCGF
基因ID : (Human) 3558
基因符号 : IL2
蛋白别名 : Aldesleukin, IL-2, T-cell growth factor, TCGF, il 2, T-cell growth factor (TCGF), Involved in regulation of T cell clonal expansion, H-IL-2, M-IL-2, ILN, Interleukin, Interleukin2, Interleukin-2, involved in regulation of T-cell clonal expansion, T cell growth factor
UniProt ID (Human) P60568
