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产品规格
检测灵敏度
1.3 pg/mL
测定范围
7,8-500
检测样本类型/体积
{Serum=50 µL, Supernatant=50 µL, Plasma=50 µL}
总时间
3 hr 10 min
均相（无需洗涤）
No
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Coated Microwell Strips
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
运输条件
Wet ice
储存
2-8°C
蛋白名称
IL-4
蛋白家族
Hormones & Receptors
均相（无需洗涤）
Human
Species (reported)
Human
检测试剂盒格式
Basic ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human Interleukin-4 (Hu IL-4) ELISA kit is a powerful tool for accurately quantifying Hu IL-4 in a variety of biological samples, including human serum, plasma, urine, or cell culture medium. This highly specific assay is designed to exclusively recognize and measure both endogenous and recombinant forms of Hu IL-4.
原理方法
The Human IL-4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) employs a meticulously selected pair of antibodies to capture and measure the target analyte. Within the microplate wells, a specific antibody that binds specifically to the target is pre-coated. Upon the addition of samples, standards, or controls, they bind to the immobilized (capture) antibody within the wells. The formation of a sandwich complex is achieved by introducing a second (detector) antibody. Subsequently, a substrate solution is added, initiating a reaction with the enzyme-antibody-target complex, resulting in the generation of a measurable signal. The intensity of this signal is directly proportional to the concentration of the target present in the original specimen.
靶标信息
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : BCGF-1, BCGF1, BSF-1, BSF1, IL-4, IL4
基因ID : (Human) 3565
基因符号 : IL4
蛋白别名 : B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, Binetrakin, BSF-1, H-IL-4, MGC79402, M-IL-4, ILN, Interleukin, Interleukin4, IL-4, interleukin 4 variant 2, Interleukin-4, Lymphocyte stimulatory factor 1, Pitrakinra
UniProt ID (Human) P05112
