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产品规格
检测灵敏度
2 pg/mL
测定范围
15,6-1000
检测样本类型/体积
{Amniotic Fluid=50 µL, Serum=50 µL, Supernatant=50 µL, Plasma=50 µL}
总时间
3 hr 10 min
均相（无需洗涤）
No
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Coated Microwell Strips
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
运输条件
Wet ice
储存
2-8°C
蛋白名称
IL-8
蛋白家族
Hormones & Receptors
均相（无需洗涤）
Human
Species (reported)
Human
检测试剂盒格式
Basic ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human Interleukin-8 (Hu IL-8) ELISA kit offers a precise method for quantifying Hu IL-8 in diverse biological samples, including human serum, plasma, amniotic fluid, or cell culture medium. This highly specific assay is designed to exclusively recognize and measure both native and recombinant forms of Hu IL-8.
原理方法
The Human IL-8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) employs a meticulously selected pair of antibodies to capture the target analyte and quantitate its amount. Within the microplate wells, a target-specific antibody is pre-coated, facilitating the binding of the analyte during the assay. Upon the introduction of samples or standards they selectively bind to the immobilized (capture) antibody within the wells. The formation of a sandwich complex is achieved by adding a second (detector) antibody. Subsequently, a substrate solution is added, initiating a reaction with the enzyme-antibody-target complex, resulting in the generation of a measurable signal. The intensity of this signal is directly proportional to the concentration of the target present in the original specimen.
靶标信息
Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.
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生物信息学
基因别名 : CXCL8, GCP-1, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1
基因ID : (Human) 3576
基因符号 : CXCL8
蛋白别名 : (Ala-IL-8)77, (Ser-IL-8)72, alveolar macrophage chemotactic factor I, ANX7, RP11-537A6.8, SNX, SYNEXIN, ILN, Interleukin, Interleukin8, beta endothelial cell-derived neutrophil activating peptide, beta-thromboglobulin-like protein, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, GCP-1, GCP/IL-8 protein I, GCP/IL-8 protein II, GCP/IL-8 protein III, GCP/IL-8 protein IV, GCP/IL-8 protein V, GCP/IL-8 protein VI, Granulocyte chemotactic protein 1, IL-8, IL-8(1-77), IL-8(5-77), IL-8(6-77), IL-8(7-77), IL-8(8-77), IL-8(9-77), IL8/NAP1 form I, IL8/NAP1 form II, IL8/NAP1 form III, IL8/NAP1 form IV, IL8/NAP1 form V, IL8/NAP1 form VI, interleukin 8, Interleukin-8, lung giant cell carcinoma-derived chemotactic protein, lymphocyte derived neutrophil activating peptide, Lymphocyte-derived neutrophil-activating factor, LYNAP, MDNCF, MDNCF-a, MDNCF-b, MDNCF-c, MONAP, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, NAF, NAP-1, Neutrophil-activating factor, neutrophil-activating peptide 1, Neutrophil-activating protein 1, Protein 3-10C, small inducible cytokine subfamily B, member 8, T-cell chemotactic factor, tumor necrosis factor-induced gene 1
UniProt ID (Human) P10145
