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产品规格
检测灵敏度
0.12 ng/mL
测定范围
0.16-10.0 ng/mL
检测样本类型/体积
{Cell Lysate=10 µL}
手工操作时间
15 min
总时间
3 hr 10 min
均相（无需洗涤）
No
批间差异
5.4%
批内差异
7.7%
检测仪器
Colorimetric Microplate Reader
产品规格
128 Tests
产品成分
Pre-coated 96 well Microplate
Detection Antibody
Anti-IgG-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
Detection Antibody
Anti-IgG-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
运输条件
Wet ice
储存
-20°C
蛋白名称
Caspase 3
均相（无需洗涤）
Human
检测试剂盒格式
Instant ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human Caspase 3(Hu Casp 3) ELISA quantitates Hu Caspase 3 in human cell extracts. The assay will exclusively recognize both natural and recombinant Hu Caspase 3.
原理方法
The Human Caspase 3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : CASP3, CPP32, CPP32B, SCA-1
基因ID : (Human) 836
基因符号 : CASP3
蛋白别名 : Apopain, Apopain precursor, Caspase3, ICE3, CASP-3, caspase 3, apoptosis-related cysteine peptidase, apoptosis-related cysteine protease, Caspase-3, Caspase-3 subunit p12, Caspase-3 subunit p17, CPP-32, Cysteine protease CPP32, PARP cleavage protease, procaspase3, Protein Yama, SCA-1, SREBP cleavage activity 1
UniProt ID (Human) P42574
