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产品规格
检测灵敏度
0.05 ng/mL
测定范围
0.08-5.0 ng/mL
检测样本类型/体积
{Cell Lysate=5 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 10 min
均相（无需洗涤）
No
批间差异
4%
批内差异
6%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plates
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Lysis Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Lysis Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
Cytochrome C
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Cytochrome c ELISA quantitates Hu cytochrome c in human cell lysates. The assay will exclusively recognize both natural and recombinant Hu cytochrome c.
原理方法
The Human cytochrome c solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : CYC, CYCS, HCS, THC4
基因ID : (Human) 54205
基因符号 : CYCS
蛋白别名 : CRF, hc1, Cytochrome c
UniProt ID (Human) P99999
