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产品规格
检测灵敏度
2 pg/mL
测定范围
1.1-800 pg/mL
检测样本类型/体积
{Plasma=33 µL, Serum=33 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
GDF15
蛋白家族
Growth Factors & Receptors
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human GDF15 ELISA quantitates Hu GDF15 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu GDF15.
原理方法
The Human GDF15 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
GDF-15 belongs to the TGF-beta cytokine family, whose members play an important role during prenatal development and postnatal growth, and the remodeling and maintenance of a variety of tissues and organs. GDF-15 is expressed predominantly in the placenta and, to a much lesser extent, in various other tissues. The presence of GDF-15 in amniotic fluid and its elevated levels in the sera of pregnant women suggest GDF-15's involvement in gestation and embryonic development. GDF-15 generally exerts tumor suppressive activities and is one of the predominant factors produced and secreted in response to activation of the p53 pathway. Interestingly, the serum level of GDF-15 is positively correlated with neoplastic progression of several tumor types, including certain colorectal, pancreatic, and prostate cancers.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : GDF-15, GDF15, MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB
基因ID : (Human) 9518
基因符号 : GDF15
蛋白别名 : GDF-15, Growth/differentiation factor 15, Macrophage inhibitory cytokine 1, MIC-1, NAG-1, NAG1, NRG-1, NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1, NSAID-activated gene 1 protein, NSAID-regulated gene 1 protein, Placental bone morphogenetic protein, Placental TGF-beta, Prostate differentiation factor, PTGF-beta
UniProt ID (Human) Q99988
