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产品规格
检测灵敏度
0.052 ng/mL
测定范围
0.156-10.0 ng/mL
检测样本类型/体积
{Cell Lysate=50 µL, Plasma=50 µL, Serum=50 µL, Supernatant=50 µL}
手工操作时间
1 hr 20 min
总时间
4 hr
均相（无需洗涤）
No
批间差异
4.5%
批内差异
5.3%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plates
Standard
Sample Diluent
Assay Buffer concentrate
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
HSPA4
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Heat Shock Protein 70 (Hu HSP70) ELISA quantitates Hu HSP70 in human serum, plasma, cell lysates or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HSP70.
原理方法
The Human HSP70 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. It has been postulated that members of the HSP 70 family act as force-generating motors, relying on the hydrolysis of ATP for their activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : APG-2, APG2, HEL-S-5a, HS24/P52, hsp70, hsp70RY, HSPA4, HSPH2, RY
基因ID : (Human) 3308
基因符号 : HSPA4
蛋白别名 : epididymis secretory sperm binding protein Li 5a, Heat shock 70 kDa protein 4, Heat shock 70-related protein APG-2, heat shock 70kD protein 4, heat shock 70kDa protein 4, heat shock protein, 110 kDa, hsp70 RY, HSP70RY, Heat shock protein
UniProt ID (Human) P34932
