Search Thermo Fisher Scientific
产品规格
检测灵敏度
2.5 pg/mL
测定范围
15.6-1,000 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL, Supernatant=50 µL}
手工操作时间
15 min
总时间
3 hr 10 min
均相（无需洗涤）
No
批间差异
8%
批内差异
7%
检测仪器
Colorimetric Microplate Reader
产品规格
128 Tests
产品成分
Pre-coated 96 well Microplate
Biotinylated Detection Antibody
SAV-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
Biotinylated Detection Antibody
SAV-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
运输条件
Wet ice
储存
-20°C
蛋白名称
TNFRSF11B
均相（无需洗涤）
Human
检测试剂盒格式
Instant ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Osteoprotegerin (TNFRSF11B) (Hu OPG) ELISA quantitates Hu OPG in human serum, plasma, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Hu OPG.
原理方法
The Human OPG solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : OCIF, OPG, PDB5, TNFRSF11B, TR1
基因ID : (Human) 4982
基因符号 : TNFRSF11B
蛋白别名 : Osteoclastogenesis inhibitory factor, Osteoprotegerin, MGC29565, soluble OPG, soluble Osteoprotegerin, sOPG, Tumor necrosis factor receptor superfamily member 11B, tumor necrosis factor receptor superfamily, member 11b
UniProt ID (Human) O00300
