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Human SAA ProQuantum Immunoassay Kit
How ProQuantum immunoassays work. (A) Antibody-antigen binding: Antibodies bind to two separate epitopes on the antigen (during a 1-hr incubation), which brings the two conjugated oligonucleotides into close proximity. (B) Ligation and amplification of signal (in a qPCR instrument): DNA ligase and a third splint oligonucleotide connector are added to ligate the two ends of the conjugated oligonucleotides, creating a 100-base DNA template. Once the ligase is inactivated at 95°C, the sample is amplified through 40 cycles of annealing and ext... View More
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产品规格
检测灵敏度
<240 pg/mL
测定范围
256 - 4,000,000 pg/mL
检测样本类型/体积
{Serum=2-5 µL, Plasma=2-5 µL, Supernatant=2-5 µL}
手工操作时间
30 min
总时间
2 hr
均相（无需洗涤）
Yes
检测仪器
qPCR Instruments
产品规格
96 Tests
产品成分
Assay Dilution Buffer
Antibody-Conjugate A
Antibody-Conjugate B
Antibody-Conjugate Dilution Buffer
Standard
Master Mix
Ligase
Antibody-Conjugate A
Antibody-Conjugate B
Antibody-Conjugate Dilution Buffer
Standard
Master Mix
Ligase
运输条件
Dry ice
储存
-20°C
蛋白名称
SAA
均相（无需洗涤）
Human
检测试剂盒格式
Proximity-based Immunoassay
标签或染料
FAM™
关于本试剂盒
The ProQuantum Human SAA Immunoassay Kit is designed to provide quantitative measurements of human serum amyloid A (SAA) in small sample volumes. Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive Cloud-based software for analytics enables sample-to-answer in just 2 hours.
• High sensitivity-detect low levels of protein with greater sensitivity than traditional methods
• Broad dynamic range-5 logarithmic units, minimizing sample dilutions to ensure they fall within the range
• Small sample consumption-use 2-5 µL of sample (compared to 75 µL for triplicate wells with other methods)
• Fast, easy workflow-2 hours from sample to answer, with no wash steps
• No proprietary instrument to purchase-runs on any real-time PCR instrument
ProQuantum immunoassays utilize a matched pair of target-specific antibodies, each conjugated to a DNA oligonucleotide. During antibody-analyte binding, the two DNA oligos are brought into close proximity, which allows for ligation of the two strands and subsequent creation of a template strand for amplification. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology (Figure 1).
The assay workflow is fast and easy-2 steps in 2 hours. There are a total of 7 components in each kit (Figure 2). First, mix the antibody-conjugates, dilute the sample, and create the standard curve in a working plate. Then, using a multi-channel pipette, add the antibody-conjugates and sample (or standard) into the wells of a PCR plate and incubate for 1 hour. Combine the master mix and ligase and add to the wells of the PCR plate, then run the plate on any qPCR instrument. After the run is complete, import the results file into the ProQuantum cloud-based software at https://apps.thermofisher.com/apps/proquantum. Using this software, the data can be analyzed easily to obtain protein concentration values. The software allows you to set up standard curves, design plate layouts, set up customized assay instructions, and obtain robust statistical group-wise comparisons.
靶标信息
The serum amyloid A family comprises a number of differentially expressed apolipoproteins, acute-phase SAA1 and SAA2, the former being the major component in plasma and constitutive SAAs. Although the liver is the primary site of synthesis of both SAA types extrahepatic production has been reported. The in vivo concentrations increase by as much as 1000 fold during inflammation. Several studies have expressed its importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations; SAA rises earlier and more sharply than CRP. Recently, a broader view of SAA expression and function has been emerging.
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生物信息学
基因别名 : PIG4, SAA, SAA1, SAA2, TP53I4
基因ID : (Human) 6288
基因符号 : SAA1
蛋白别名 : Amyloid fibril protein AA, Amyloid protein A, MGC111216, amyloid a, Serum Amyloid-A, Serum amyloid A1 protein, SAA, serum amyloid A protein, Serum amyloid A-1 protein, Serum amyloid protein A(2-102), Serum amyloid protein A(2-103), Serum amyloid protein A(2-104), Serum amyloid protein A(3-104), Serum amyloid protein A(4-101), tumor protein p53 inducible protein 4
UniProt ID (Human) P0DJI8
