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产品规格
检测灵敏度
4.6 pg/mL
测定范围
15.6-1,000 pg/mL
检测样本类型/体积
{Plasma=100 µL, Serum=100 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 10 min
均相（无需洗涤）
No
批间差异
10.2%
批内差异
8%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
TNF beta
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human Tumor Necrosis Factor beta (Hu TNFβ) ELISA quantitates Hu TNFβ in human serum, plasma, urine, synovial fluid, amniotic fluid, cell culture supernatants, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu TNFβ.
原理方法
The Human TNFβ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : LT, LTA, TNFB, TNFSF1, TNLG1E
基因ID : (Human) 4049
基因符号 : LTA
蛋白别名 : LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, DAMA-25N12.13, TNF beta, TNF b, TNF β, TNFbeta, TNFβ, Lymphotoxin-alpha, TNF superfamily, member 1, tumor necrosis factor beta, tumor necrosis factor ligand 1E
UniProt ID (Human) Q9UKS8
