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产品规格
检测灵敏度
0.03 ng/mL
测定范围
0.6-40.0 ng/mL
检测样本类型/体积
{Plasma=20 µL, Serum=20 µL, Supernatant=20 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 30 min
均相（无需洗涤）
No
批间差异
5%
批内差异
5.7%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
VEGF Receptor 3
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Vascular Endothelial Growth Factor Receptor 3/FLT4 (Hu VEGFR3) ELISA quantitates Hu VEGFR3 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu VEGFR3.
原理方法
The Human VEGFR3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Vascular endothelial growth factor is a key regulator of blood vessel development in embryos and angiogenesis in adult tissues. Unlike VEGF, the related VEGFC stimulates the growth of lymphatic vessels through its specificl ymphatic endothelial receptor VEGFR-3. In 1998, Dumont showed that targeted inactivation of the VEGFR-3 gene in mice resulted in defective blood vessel development in early embryos. Vasculogenesis and angiogenesis occurred, but large vessels became abnormally organized with defective lumens, leading to fluid accumulation in the pericardial cavity and cardiovascular failure at embryonic day 9.5. Thus, VEGFR3 has an essential role in the development of theembryonic cardiovascular system before the emergence of the lymphatic vessels.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : FLT-4, FLT4, FLT41, LMPH1A, PCL, VEGFR-3, VEGFR3
基因ID : (Human) 2324
基因符号 : FLT4
蛋白别名 : FLT-4, Fms-like tyrosine kinase 4, fms-related tyrosine kinase 4, Tyrosine-protein kinase receptor FLT4, Vascular endothelial growth factor receptor 3, VEGFR-3, sVEGFR 3, VEGF R3, soluble VEGFR 3
UniProt ID (Human) P35916
