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产品规格
检测灵敏度
<5 pg/mL
测定范围
7.81-500 pg/mL
检测样本类型/体积
{Cerebrospinal Fluid=10 µL, Supernatant=10 µL, Tissue Homogenate=10 µL}
手工操作时间
1 hr 20 min
总时间
4 hr
均相（无需洗涤）
No
批间差异
4.8%
批内差异
5%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Standard Dilution Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
Amyloid beta 40
均相（无需洗涤）
Mouse
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Mouse Amyloid Beta 42 (Ms Abeta 42) ELISA quantitates Ms Abeta 42 in human cerebrospinal fluid, tissue homogenates and culture supernatant. The assay will exclusively recognize both natural and recombinant Ms Abeta 42.
原理方法
The Mouse Abeta 42 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Beta-amyloid is a 40-43 amino acid peptide cleaved from amyloid precursor protein, and a putative gamma-secretase. Amyloid beta (1-40) together with Amyloid beta (1-42) are two major C-terminal variants of the amyloid beta protein, constituting the majority of amyloid beta proteins. Both the amyloid beta (40) and amyloid beta (42) alloforms are major components of the extracellular plaques found in Alzheimer's disease brain tissue.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : A4, Abeta, Abpp, AD1, Adap, Ag, App, betaApp, Cvap, E030013M08Rik
基因ID : (Mouse) 11820
基因符号 : App
蛋白别名 : Abeta40, Abeta42, ABPP, AG, AID(50), AID(57), AID(59), Alpha-CTF, Alpha-secretase C-terminal fragment, Alzheimer disease amyloid A4 protein homolog, Alzheimer disease amyloid protein, amyloid beta A4 protein, Amyloid intracellular domain 50, Amyloid intracellular domain 57, Amyloid intracellular domain 59, Amyloid precursor protein, Amyloid, Amyloid b, Amyloid beta, Amyloid β, B APP40, BAPP40, BetaAPP40, ß APP40, ßAPP40, A beta 1 40, A ß 1 40, AB40, Amyloid beta 1 40, Aß 1 40, Amyloid-beta (A4) precursor protein, Amyloid-beta A4 protein, Amyloid-beta precursor protein, Amyloid-beta protein 40, Amyloid-beta protein 42, Amyloidogenic glycoprotein, APP, APP-C57, APP-C59, APP-C99, appican, Beta-APP40, Beta-APP42, Beta-CTF, Beta-secretase C-terminal fragment, C31, C80, C83, C99, Gamma-CTF(50), Gamma-CTF(57), Gamma-CTF(59), Gamma-secretase C-terminal fragment 50, Gamma-secretase C-terminal fragment 57, Gamma-secretase C-terminal fragment 59, N-APP, P3(40), P3(42), protease nexin II, S-APP-alpha, S-APP-beta, Soluble APP-alpha, Soluble APP-beta
UniProt ID (Mouse) P12023
