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The Mouse Complement Component 3a (C3a) ELISA quantitates C3a in serum, plasma, and other biological fluids
The Mouse Complement Component 3a (C3a) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : AI255234, ASP, C3, HSE-MSF, Plp
基因ID : (Mouse) 12266
基因符号 : C3
蛋白别名 : Acylation stimulating protein, ASP, C3-beta-c, C3a anaphylatoxin, C3adesArg, C3bc, C3r1, Complement C3, Complement C3 alpha chain, Complement C3 beta chain, Complement C3b alpha' chain, Complement C3c alpha' chain fragment 1, Complement C3c alpha' chain fragment 2, Complement C3d fragment, Complement C3dg fragment, Complement C3f fragment, Complement C3g fragment, HSE-MSF
UniProt ID (Mouse) P01027