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产品规格
检测灵敏度
4 pg/mL
测定范围
4-500 pg/mL
检测样本类型/体积
{Serum=100 µL, Supernatant=100 µL}
手工操作时间
1 hr 45 min
总时间
24 hr 30 min
均相（无需洗涤）
No
检测仪器
Colorimetric Microplate Reader
产品规格
2 x 96 Tests
产品成分
Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, HRP-conjugated antibody
Standard: Recombinant protein for generating standard curve and calibrating samples
10X Coating Buffer: Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme
Substrate: 1X TMB Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Detection Antibody: Pre-titrated, HRP-conjugated antibody
Standard: Recombinant protein for generating standard curve and calibrating samples
10X Coating Buffer: Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme
Substrate: 1X TMB Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
运输条件
Wet ice
储存
2-8°C
蛋白名称
IL-13
均相（无需洗涤）
Mouse
检测试剂盒格式
Uncoated ELISA Kit with Plates
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Mouse Interleukin 13 (IL-13) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-13. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.
原理方法
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
靶标信息
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : Il-13, Il13
基因ID : (Mouse) 16163
基因符号 : Il13
蛋白别名 : IL-13, T-cell activation protein P600, ALRH, ALRH antibody, BHR1, MGC116786, Interleukin13, il 13, MGC116788, MGC116789, NC30 antibody, P600 antibody, ILN, Interleukin, Interleukin-13
UniProt ID (Mouse) P20109
