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产品规格

检测灵敏度

<0.03 ng/mL

测定范围

0.032- 2500 ng/mL

检测样本类型/体积

{Serum=2-5 µL, Plasma=2-5 µL, Supernatant=2-5 µL}

手工操作时间

30 min

总时间

2 hr

均相（无需洗涤）

Yes

检测仪器

qPCR Instruments

产品规格

96 Tests

产品成分

Assay Dilution Buffer
Antibody-Conjugate A
Antibody-Conjugate B
Antibody-Conjugate Dilution Buffer
Standard
Master Mix
Ligase

运输条件

Dry ice

储存

-20°C

蛋白名称

LAP

均相（无需洗涤）

Mouse

检测试剂盒格式

Proximity-based Immunoassay

标签或染料

FAM™

关于本试剂盒

The ProQuantum Mouse LAP/TGFbeta Immunoassay Kit is designed to provide quantitative measurements of mouse LAP/TGFbeta in small sample volumes. Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive software for analytics enables sample-to-answer in just 2 hours.

• High sensitivity-detect low levels of protein with greater sensitivity than traditional methods

• Broad dynamic range-5 logarithmic units, minimizing sample dilutions to ensure they fall within the range

• Small sample consumption-use 2-5 µL of sample (compared to 75 µL for triplicate wells with other methods)

• Fast, easy workflow-2 hours from sample to answer, with no wash steps

• No proprietary instrument to purchase-runs on any real-time PCR instrument

ProQuantum immunoassays utilize a matched pair of target-specific antibodies, each conjugated to a DNA oligonucleotide. During antibody-analyte binding, the two DNA oligos are brought into close proximity, which allows for ligation of the two strands and subsequent creation of a template strand for amplification. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology (Figure 1).

The assay workflow is fast and easy-2 steps in 2 hours. There are a total of 7 components in each kit (Figure 2). First, mix the antibody-conjugates, dilute the sample, and create the standard curve in a working plate. Then, using a multi-channel pipette, add the antibody-conjugates and sample (or standard) into the wells of a PCR plate and incubate for 1 hour. Combine the master mix and ligase and add to the wells of the PCR plate, then run the plate on any qPCR instrument. After the run is complete, import the results file into the ProQuantum software (cloud-based version at https://apps.thermofisher.com/apps/proquantum). Using this software, the data can be analyzed easily to obtain protein concentration values. The software allows you to set up standard curves, design plate layouts, apply a 5PL weighted algorithm, and obtain robust statistical group-wise comparisons.

靶标信息

TGF beta-1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its prodomain. Activation of TGF-b1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF-beta, an aminoterminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

基因别名 : TGF-beta1, Tgfb, Tgfb-1, Tgfb1, TGFbeta1

基因ID : (Mouse) 21803

基因符号 : Tgfb1

蛋白别名 : LAP, Latency-associated peptide, regulatory protein, TGF-beta 1, TGF-beta-1, Transforming growth factor beta-1, Transforming growth factor beta-1 proprotein, transforming growth factor-beta 1

UniProt ID (Mouse) P04202

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