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_web-data.jpg?time=20240412 "Multispecies AKT (Total/Phospho) InstantOne™ ELISA Kit")
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Multispecies AKT (Total/Phospho) InstantOne™ ELISA Kit
Representative data for AKT (Total/Phospho) Multispecies InstantOne™ ELISA. MCF7 cells were pre-treated with 10 µM wortmannin washed and unstimulated (-) or stimulated with 10 ng/mL insulin for 10 min (+). Cells were analyzed for total AKT 1 (left) or phosphorylated AKT 1/2/3 (Ser473) (right) using the AKT (Total/Phospho) InstantOne™ ELISA.
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产品规格
检测样本类型/体积
{Cell Lysate=20 µL}
手工操作时间
1 hr 20 min
总时间
1 hr 10 min
均相（无需洗涤）
No
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
运输条件
Wet ice
储存
2-8°C
蛋白名称
AKT Pan
Target sub-specificity
Total
均相（无需洗涤）
Human
检测试剂盒格式
InstantOne ELISA
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The InstantOne™ ELISA is specifically engineered for accurate measurement of human, mouse, and hamster total AKT 1 and phosphorylated AKT 1/2/3 (Ser473) in cell lysates. Detection of rat AKT 1 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
靶标信息
AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : AKT, AKT1, AKT2, AKT3, CWS6, HIHGHH, MPPH, MPPH2, PKB, PKB-ALPHA, PKB-GAMMA, PKBB, PKBBETA, PKBG, PRKBA, PRKBB, PRKBG, RAC, RAC-ALPHA, RAC-BETA, RAC-gamma, RAC-PK-gamma, STK-2
基因ID : (Human) 207, (Human) 208, (Human) 10000
基因符号 : AKT1, AKT2, AKT3
蛋白别名 : AKT1m, DKFZp434N0250, kinase Akt1, MGC99656, pan akt, murine thymoma viral (v-akt) homolog-2, PKB, PKB alpha, PKB beta, PKB gamma, Protein kinase Akt-2, Protein kinase Akt-3, Protein kinase B, Protein kinase B alpha, Protein kinase B beta, Protein kinase B gamma, Proto-oncogene c-Akt, putative v-akt murine thymoma viral oncoprotein 2, rac protein kinase alpha, rac protein kinase beta, RAC-alpha serine/threonine-protein kinase, RAC-beta serine/threonine-protein kinase, RAC-gamma serine/threonine protein kinase, RAC-gamma serine/threonine-protein kinase, RAC-PK-alpha, RAC-PK-beta, RAC-PK-gamma, serine-threonine protein kinase, STK-2, v-akt murine thymoma viral oncogene homolog 1, v-akt murine thymoma viral oncogene homolog 2, v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma), v-akt murine thymoma viral oncogene-like protein 1
UniProt ID (Human) P31749, (Human) P31751, (Human) Q9Y243
