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![Multispecies STAT3 (Phospho) [pY705] ELISA Kit](https://qa1.thermofisher.cn/antibody/images/650/KHO0481_SC_080324.jpg?time=20240412 "Multispecies STAT3 (Phospho) [pY705] ELISA Kit")
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![Multispecies STAT3 (Phospho) [pY705] ELISA Kit (KHO0481)](https://qa1.thermofisher.cn/antibody/images/150/Sandwich-Direct-ELISA-Kit-Packaging-650x600.jpg?time=20240412)
产品规格
检测灵敏度
<0.9 U/mL
测定范围
1.57-100 U/mL
检测样本类型/体积
{Cell Lysate=10 µL}
手工操作时间
1 hr 20 min
总时间
4 hr
均相（无需洗涤）
No
批间差异
4.5%
批内差异
3.9%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
STAT3
Target site-specificity
Phosphorylated Tyr705
蛋白家族
Transcription Factors
均相（无需洗涤）
Human, Mouse, Rat
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human STAT3 (Phospho) [pY705] ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of STAT3 (Phospho) [pY705] in fresh or frozen human cell lysates. Cross-reactivity has been observed in mouse and rat cells. The assay recognizes both natural and recombinant human STAT3 (Phospho) [pY705].
原理方法
A monoclonal capture antibody specific for STAT3 (Phospho) [pY705]) has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described. Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : 1110034C02Rik, ADMIO, ADMIO1, APRF, AW109958, HIES, STAT3
基因ID : (Human) 6774, (Rat) 25125, (Mouse) 20848
基因符号 : STAT3, Stat3, Stat3
蛋白别名 : acute phase response factor, Acute-phase response factor, FLJ20882, MGC16063, STAT, DNA-binding protein APRF, Signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase response factor)
UniProt ID (Human) P40763, (Rat) P52631, (Mouse) P42227
