Search Thermo Fisher Scientific
产品规格
检测灵敏度
1 pg/mL
测定范围
1.56-100 pg/mL
检测样本类型/体积
{Tissue Homogenate=50 µL, Serum=50 µL, Cell Lysate=50 µL, Plasma=50 µL}
手工操作时间
1 hr 20 min
总时间
2 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
Estradiol
均相（无需洗涤）
Pig, Rat
Species (reported)
Pig, Rat
检测试剂盒格式
Competitive ELISA
检测抗体结合物
Biotin
标签或染料
Unconjugated
关于本试剂盒
The Rat/Porcine Estradiol (E2) ELISA quantitates E2 in serum and plasma.
原理方法
The Competitive Thromboxane B2 (TXB2) ELISA research use only kit is designed to quantitatively measure TXB2 in Humans. A TXB2 standard is provided to generate a standard curve for the assay and all samples are read off a user generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Human TXB2 is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Human TXB2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Estradiol (17 beta-estradiol) is a sex hormone. Estradiol is the predominant sex hormone present in females. It is also present in males, being produced as an active metabolic product of testosterone. It represents the major estrogen in humans. Estradiol has not only a critical impact on reproductive and sexual functioning, but also affects other organs including the bones.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名 : 17-Beta-Estradiol, 17-beta-HSD
