Invitrogen

CD4 Monoclonal Antibody (SK3 (SK-3)), FITC, eBioscience™

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CD4 Antibody (11-0047-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD8a APC (Product # 17-0088-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD4 FITC (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

11-0047-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.06 µg)/test

查看14篇已发表文章 14篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

SK3 (SK-3)

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1272074

产品详细信息

Description: The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T helper cells, and, in low levels, monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). SK3 blocks HIV binding and mixed lymphocyte reaction. The SK3 and RPA-T4 monoclonal antibodies do not cross-block binding, suggesting recognition of distinct epitopes.

Applications Reported: This SK3 (SK-3) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SK3 (SK-3) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

Abnormally high expression of D1-like dopamine receptors on lupus CD4⁺ T cells promotes Tfh cell differentiation.

Lupus science & medicine

Wu F,Xiang Z,He Z,Yi P,Yang M,Wu H,Hu M

11-0047-42 was used in Flow cytometry/Cell sorting to explore the relationship between DRDs and Tfh cells, and analyse the relationship between DRD expression on Tfh cells and the course of SLE.

Tue Aug 01 00:00:00 UTC 2023

SPARC regulation of PMN clearance protects from pristane-induced lupus and rheumatoid arthritis.

iScience

Sangaletti S,Botti L,Gulino A,Lecis D,Bassani B,Portararo P,Milani M,Cancila V,De Cecco L,Dugo M,Tripodo C,Colombo MP

Published figure using CD4 monoclonal antibody (Product # 11-0047-42) in Flow Cytometry

Fri Jun 25 00:00:00 UTC 2021

Single-cell multi-omics analysis presents the landscape of peripheral blood T-cell subsets in human chronic prostatitis/chronic pelvic pain syndrome.

Journal of cellular and molecular medicine

Zhang M,Liu Y,Chen J,Chen L,Meng J,Yang C,Yin S,Zhang X,Zhang L,Hao Z,Chen X,Liang C

11-0047 was used in Flow cytometry/Cell sorting to digitally quantify both the protein and mRNA expression levels in single peripheral blood T cells from two chronic prostatitis / chronic pelvic pain patients and two healthy controls.

Tue Dec 01 00:00:00 UTC 2020

Interleukin-6-knockdown of chimeric antigen receptor-modified T cells significantly reduces IL-6 release from monocytes.

Experimental hematology & oncology

Kang L,Tang X,Zhang J,Li M,Xu N,Qi W,Tan J,Lou X,Yu Z,Sun J,Wang Z,Dai H,Chen J,Lin G,Wu D,Yu L

Published figure using CD4 monoclonal antibody (Product # 11-0047-42) in Flow Cytometry

Fri Apr 15 00:00:00 UTC 2022

Cytotoxic effects of ex vivo-expanded natural killer cell-enriched lymphocytes (MYJ1633) against liver cancer.

BMC cancer

Choi JW,Lee ES,Kim SY,Park SI,Oh S,Kang JH,Ryu HA,Lee S

11-0047-42 was used in Flow Cytometry to show that the house-developed method can be a new and effective strategy to prepare clinically applicable NKL for autologous NK cell-based anti-tumor immunotherapy.

Mon Aug 19 00:00:00 UTC 2019

Human P2Y₁₁ Expression Level Affects Human P2X7 Receptor-Mediated Cell Death.

Frontiers in immunology

Dreisig K,Sund L,Dommer MW,Kristensen NP,Boddum K,Viste R,Fredholm S,Odum N,Jäättelä M,Skov S,Kornum BR

11-0047 was used in Flow cytometry/Cell sorting to show that in human T lymphocytes, the expression of the human P2Y11 receptor correlates with human P2X7 receptor-driven cell death following BzATP stimulation.

Fri Aug 16 00:00:00 UTC 2019

The Genetic Polymorphisms of NLRP3 Inflammasome Associated with T Helper Cells in Patients with Multiple Myeloma.

Journal of immunology research

Zhao X,Hua M,Yan S,Yu J,Han F,Zhong C,Wang R,Zhang C,Hou M,Ma D

11-0047 was used in Flow cytometry/Cell sorting to investigate the role of the NLRP3 inflammasome in multiple myeloma.

Fri Nov 09 00:00:00 UTC 2018

Characteristic patterns of HLA presentation and T cell differentiation in adult-onset Still's disease.

International journal of immunopathology and pharmacology

Jung JY,Choi B,Sayeed HM,Suh CH,Kim YW,Kim HA,Sohn S

Published figure using CD4 monoclonal antibody (Product # 11-0047-42) in Flow Cytometry

Tue Dec 18 00:00:00 UTC 2018

HDAC inhibition potentiates immunotherapy in triple negative breast cancer.

Oncotarget

Terranova-Barberio M,Thomas S,Ali N,Pawlowska N,Park J,Krings G,Rosenblum MD,Budillon A,Munster PN

Published figure using CD4 monoclonal antibody (Product # 11-0047-42) in Flow Cytometry

Tue Dec 26 00:00:00 UTC 2017

Dinitrophenyl hapten with laser immunotherapy for advanced malignant melanoma: A clinical study.

Oncology letters

Chen DJ,Li XS,Zhao H,Fu Y,Kang HR,Yao FF,Hu J,Qi N,Zhang HH,Du N,Chen WR

11-0047-42 was used in Flow cytometry/Cell sorting to evaluate the efficacy and safety of in situ immunotherapy with dinitrophenyl (DNP) hapten in combination with laser therapy for patients with malignant melanoma (MM).

Wed Mar 01 00:00:00 UTC 2017

Chloroquine inhibits human CD4⁺ T-cell activation by AP-1 signaling modulation.

Scientific reports

Schmidt RL,Jutz S,Goldhahn K,Witzeneder N,Gerner MC,Trapin D,Greiner G,Hoermann G,Steiner G,Pickl WF,Burgmann H,Steinberger P,Ratzinger F,Schmetterer KG

11-0047 was used in Flow cytometry/Cell sorting to demonstrate that chloroquine has immunomodulatory effects on CD4(+) T-cells.

Tue Feb 07 00:00:00 UTC 2017

Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.

The Journal of clinical investigation

MacDonald KG,Hoeppli RE,Huang Q,Gillies J,Luciani DS,Orban PC,Broady R,Levings MK

Published figure using CD4 monoclonal antibody (Product # 11-0047-42) in Flow Cytometry

Fri Apr 01 00:00:00 UTC 2016

Effects of transcutaneous acupoint electrical stimulation on the imbalance of Th₁, Th₂, Th₁₇ and T_reg cells following thoracotomy of patients with lung cancer.

Experimental and therapeutic medicine

Wu H,Wang K,Li G,Meng D,Han J,Wang G,Li YU

11-0047 was used in Flow cytometry/Cell sorting to demonstrate that transcutaneous acupoint electrical stimulation was able to partially attenuate the postoperative immune depression of patients with lung cancer.