TRIzol™Plus RNA 纯化试剂盒
TRIzol™Plus RNA 纯化试剂盒
Invitrogen™

TRIzol™Plus RNA 纯化试剂盒

The TRIzol Plus RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a了解更多信息
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货号 12183555
价格(CNY)/ EA 50 preps
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The TRIzol Plus RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a wide variety of samples, including animal and plant cells, tissue, bacteria, and yeast. The kit combines the strong lysis capability of TRIzol Reagent (Cat. No. 15596026), followed by a convenient and time-saving silica-cartridge purification protocol from the PureLink RNA Mini Kit (Cat. No. 12183020), to provide ultrapure total RNA typically within an hour, even from difficult samples such as fibrous or fatty tissues.

Features of this kit include:

• Combined TRIzol-based lysis and PureLink spin column-based RNA isolation technologies
• Superior lysis capability and convenient protocol

Two products in one
The TRIzol Plus RNA Purification System couples the lysis capabilities of TRIzol Reagent with the 1000 μg column-;binding capacity of the PureLink RNA Mini Kit, resulting in high RNA integrity and yield. The TRIzol Plus RNA Purification System includes protocols for a variety of sample types, including fibrous or fatty tissues and plant material. The combination of TRIzol Reagent followed by silica-cartridge purification of RNA is the recommended RNA purification method in the gene expression industry. The TRIzol Plus RNA Purification Kit provides the reagents and an optimized protocol to purify total RNA for gene expression studies using an industry recommended method.

Simple lysis to column purification protocol
TRIzol Reagent sample lysis maintains the integrity of the RNA, while disrupting cells and dissolving cell components. TRIzol Reagent also provides an immediate and highly effective inhibition of RNase activity during sample homogenization. Following lysis, RNA in the lysed sample is bound to the clear silica-based membrane in the PureLink RNA Mini Kit Spin Cartridge, washed to remove contaminants, and then eluted in RNase-Free water (Tris Buffer, pH 7.5 may also be used).
仅供科研使用。不可用于诊断程序。
规格
分离技术硅胶离心柱, 有机提取
纯化时间20 min.
洗脱体积30 to 300 μL
样品类型细菌, 细胞, 植物样品, 组织, 酵母菌
最终产品类型总 RNA
高通量能力不兼容高通量应用(手动)
适用于(应用)微阵列分析、实时荧光定量 PCR (qPCR)、逆转录酶 PCR (RT-PCR)、cDNA 文库构建、核酸标记、核酸酶保护试验、Northern 印迹
数量50 Preps
规格小型
运输条件Room Temperature
原始材料量多达 5 x 10^8 个细胞,多达 200 mg 组织
产量1000 µg
Unit SizeEA 50 preps
内容与储存
试剂盒组成部分:
• TRIzol™ 试剂 (100 mL)
• 裂解缓冲液 (125 mL)
• 洗涤缓冲液 I (50 mL)
• 洗涤缓冲液 II (15 mL)
• 无 RNase 水 (15.5 mL)
• 带收集管的离心柱(各 50 个)
• 收集管(各 50 个)
• 回收管(各 50 个)

所有组分均储存在室温下。提供足够进行 50 次 RNA 分离的试剂。