Invitrogen

CX3CR1 Polyclonal Antibody, eBioscience™

CX3CR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CX3CR1 Antibody (14-6093-81) in IHC (P)

Immunohistochemical staining with antigen retreival of formalin-fixed, paraffin-embedded human heart tissue using Anti-CX3CR1 Purified at 2 µg/mL and detected using Anti-Rabbit IgG-HRP.

CX3CR1 Antibody in Immunocytochemistry (ICC/IF)

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产品信息

14-6093-81

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

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功能检测 (Functional)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_467880

产品详细信息

Description: The polyclonal rabbit antibody reacts with human, mouse, and rat Fractalkine receptor; the antibody was raised against a peptide corresponding to amino acids 2 to 21 of human Fractalkine receptor (CX3CR1), this sequence of human CX3CR1 differs from those of mouse and rat by four amino acids. CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, murine, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1 and RBS11, respectively. The encoded seven transmembrane protein was recently identified as the receptor for a novel transmembrane molecule, fractalkine, and renamed CX3CR1. Recently, CX3CR1 was found to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration and adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines.

Applications Reported: This polyclonal antibody has been reported for use in immunoblotting (WB).

Applications Tested: This polyclonal antibody has been tested by immunoblotting (1:500 to 1:2000). Human spleen tissue can be used as a positive control and an approximatley 50 kDa band can be detected. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, mouse, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively. The encoded seven transmembrane protein was recently identified as the receptor for a novel transmembrane molecule, fractalkine, and renamed CX3CR1. Recently, CX3CR1 was found to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration an d adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Phospholipid scramblase-1 regulates innate type 2 inflammation in mouse lungs via CRTH2-dependent mechanisms.

The Journal of clinical investigation

Hernandez-Gutierrez A,Majid S,Eberle A,Choi A,Sorkhdini P,Yang D,Yang AX,Norbrun C,He CH,Lee CM,Lee CG,Elias JA,Zhou Y

14-6093-81 was used in Immunohistochemistry to demonstrate that PLSCR1 played an essential role in the pathogenesis of ILC2 responses, providing critical insights into biology and disease pathogenesis and identifying targets that can be manipulated in attempts to control IT2IR in chronic diseases such as asthma.

Tue Aug 01 00:00:00 UTC 2023

Altered cell and RNA isoform diversity in aging Down syndrome brains.

Proceedings of the National Academy of Sciences of the United States of America

Palmer CR,Liu CS,Romanow WJ,Lee MH,Chun J

Published figure using CX3CR1 polyclonal antibody (Product # 14-6093-81) in Western Blot

Tue Nov 23 00:00:00 UTC 2021

PDTC ameliorates neuropathic pain by inhibiting microglial activation via blockage of the TNFα-CX3CR1 pathway.

European journal of histochemistry : EJH

Li X,Ye Z,Guo Q,Wang E,Pan Y

14-6093 was used in Western Blotting to conclude that intrathecal infusion of PDTC could attenuate the pain-related behaviors induced by sciatic nerve CCI through suppressing the spinal microglia activation and TNF-a up-regulation in rats.

Fri Mar 12 00:00:00 UTC 2021

PDTC ameliorates neuropathic pain by inhibiting microglial activation via blockage of the TNFα-CX3CR1 pathway.

European journal of histochemistry : EJH

Li X,Ye Z,Guo Q,Wang E,Pan Y

Fri Mar 12 00:00:00 UTC 2021

Modulation of inflammatory responses by fractalkine signaling in microglia.

PloS one

Inoue K,Morimoto H,Ohgidani M,Ueki T

14-6093-81 was used in Immunocytochemistry-immunoflourescence to investigate the influence of fractalkine on immunoreactive microglia using murine primary microglia and BV-2, a microglial cell line.

Tue Oct 26 00:00:00 UTC 2021

Modulation of inflammatory responses by fractalkine signaling in microglia.

PloS one

Inoue K,Morimoto H,Ohgidani M,Ueki T

14-6093-81 was used in Immunocytochemistry-immunoflourescence to investigate the influence of fractalkine on immunoreactive microglia using murine primary microglia and BV-2, a microglial cell line.

Tue Oct 26 00:00:00 UTC 2021

Germline nuclear-predominant Pten murine model exhibits impaired social and perseverative behavior, microglial activation, and increased oxytocinergic activity.

Molecular autism

Sarn N,Thacker S,Lee H,Eng C

14-6093-81 was used in Immunocytochemistry to explore the effects of PTEN localization on different neurophenotypes.

Fri Jun 04 00:00:00 UTC 2021

Germline nuclear-predominant Pten murine model exhibits impaired social and perseverative behavior, microglial activation, and increased oxytocinergic activity.

Molecular autism

Sarn N,Thacker S,Lee H,Eng C

14-6093-81 was used in Immunocytochemistry to explore the effects of PTEN localization on different neurophenotypes.

Fri Jun 04 00:00:00 UTC 2021

Acute and Persistent Alterations of Cerebellar Inflammatory Networks and Glial Activation in a Rat Model of Pediatric Mild Traumatic Brain Injury.

Journal of neurotrauma

Fraunberger EA,DeJesus P,Zanier ER,Shutt TE,Esser MJ

14-6093 was used in Western Blotting to provide insight into potential mechanisms of persistent changes that could contribute to neurological dysfunction by characterising the evolution of cerebellar inflammation in pediatric mTBI.

Mon Jun 01 00:00:00 UTC 2020

Plasmodium falciparum proteins involved in cytoadherence of infected erythrocytes to chemokine CX3CL1.

Scientific reports

Hermand P,Cicéron L,Pionneau C,Vaquero C,Combadière C,Deterre P

14-6093 was used in Western Blotting to investigate proteins produced by Plasmodium falciparum for adherence to CX3CL1.

Thu Sep 22 00:00:00 UTC 2016

Plasmodium falciparum proteins involved in cytoadherence of infected erythrocytes to chemokine CX3CL1.

Scientific reports

Hermand P,Cicéron L,Pionneau C,Vaquero C,Combadière C,Deterre P

14-6093 was used in Western Blotting to investigate proteins produced by Plasmodium falciparum for adherence to CX3CL1.

Thu Sep 22 00:00:00 UTC 2016

Liver-resident NK cells confer adaptive immunity in skin-contact inflammation.

The Journal of clinical investigation

Peng H,Jiang X,Chen Y,Sojka DK,Wei H,Gao X,Sun R,Yokoyama WM,Tian Z

14-6093 was used in Flow cytometry/Cell sorting to define the transcriptional, phenotypic and functional features of natural killer cells in the liver; showing that liver resident CD49a+DX5- natural killer cells possess memory potential and are derived from HPCs/HSCs rather than bone marrow.

Mon Apr 01 00:00:00 UTC 2013

Human renal cell carcinoma induces a dendritic cell subset that uses T-cell crosstalk for tumor-permissive milieu alterations.

The American journal of pathology

Figel AM,Brech D,Prinz PU,Lettenmeyer UK,Eckl J,Turqueti-Neves A,Mysliwietz J,Anz D,Rieth N,Muenchmeier N,Buchner A,Porubsky S,Siegert SI,Segerer S,Nelson PJ,Noessner E

14-6093 was used in Flow cytometry/Cell sorting to investigate the interaction of dendritic cells and T cells in human renal cell carcinoma.

Fri Jul 01 00:00:00 UTC 2011

Fractalkine is expressed in the human ovary and increases progesterone biosynthesis in human luteinised granulosa cells.

Reproductive biology and endocrinology : RB&E

Huang S,Zhao P,Yang L,Chen Y,Yan J,Duan E,Qiao J

14-6093 was used in Immunohistochemistry to provide the first detailed report of fractalkine and CX3CR1 expression and function in the human ovary.

Thu Jun 30 00:00:00 UTC 2011

生物信息学

蛋白别名: Beta chemokine receptor-like 1; C-X3-C CKR-1; chemokine (C-C) receptor-like 1; chemokine (C-X3-C motif) receptor 1; chemokine (C-X3-C) receptor 1; CMK-BRL-1; CMK-BRL1; Cx3c; CX3C chemokine receptor 1; Fractalkine receptor; G protein-coupled receptor 13; G-protein coupled receptor 13; Scyd1; V28

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基因别名: CCRL1; CMKBRL1; CMKDR1; CX3CR1; GPR13; GPRV28; Rbs11; V28

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UniProt ID: (Human) P49238, (Rat) P35411, (Mouse) Q9Z0D9

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Entrez Gene ID: (Human) 1524, (Rat) 171056, (Mouse) 13051

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功能

chemokine receptor activity protein binding C-X3-C chemokine receptor activity C-X3-C chemokine binding cytokine receptor activity signal transducer activity G-protein coupled receptor activity

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