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cGAS Antibody (703149) in WB
Western blot analysis was performed on whole Cell extracts (30 µg lysate) of RAW 264.7 (Lane 1), RAW 264.7 transfected with poly dA:dT (1.33 µg/mL for 16 hrs) (Lane 2) and NIH/3T3 (Lane 3). The blot was probed with Anti-cGAS (Mouse) Recombinant Rabbit Monoclonal Antibody (Product # 703149, 1:1,000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4,000 dilution). A 59 kDa band corresponding to cGAS (Mouse) was observed across the cell lin... View More
产品信息
703149
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
10H1L5
抗原
Protein corresponding to mouse cGAS [aa147-aa507]
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2809227
产品详细信息
This antibody is predicted to react with Monkey, Rat, Pig.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
cGAS (Cyclic GMP-AMP synthase) is a nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and plays a key role in innate immunity. Catalysis involves both the formation of 2', 5' phosphodiester linkage at the GpA step and the formation of 3', 5' phosphodiester linkage at the ApG step. This produces c[G(2',5')pA(3'5')p] responses. cGAS also binds cytosolic DNA directly, leading to the activation and synthesis of cGAMP. Then, a second messenger binds to and activates TMEM173/STING thereby triggering type-I interferon production. cGAS is also involved in innate immune sensorship of infection, detection of DNA from bacteria, response to cellular stress, DNA damage, and regulation of cellular senescence.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 2'3'-cGAMP synthase; C6orf150; cGAMP synthase; Cyclic GMP-AMP synthase; h-cGAS; Mab-21 domain-containing protein 1; MB21D1
基因别名: Cgas; E330016A19Rik; Mb21d1
UniProt ID: (Mouse) Q8C6L5
Entrez Gene ID: (Mouse) 214763
