Axiom™ PangenomiX Array Kit

The Axiom™ PangenomiX genotyping array offers ethnically diverse genomic coverage in a high throughput format array. This human genotyping research array was designed for whole genome imputation across global populations, enabling scientists to create diverse genomic datasets that have traditionally been built on Caucasian cohorts. It is a powerful research tool in human genomics, including applications such as genome wide association studies (GWAS), population health initiatives, polygenic risk scores, and drug discovery.

The Axiom PangenomiX Array can be used to analyze the whole genome, from as little as 100 ng genomic DNA, and enable target SNP identification, copy number variant (CNV) analysis, human leukocyte antigen (HLA) and blood typing in a cost-effective assay with plug and play data analysis.

More than 850,000 markers were selected for high genomic coverage from the 1000 Genomes Project Phase III, yielding coverage for European, African, admixed American, East Asian, and South Asian populations. This means variants prevalent in different populations are represented, leading to more inclusive research outcomes.

In addition to markers selected specifically to maximize imputation power across ethnicities, markers were chosen from broadly referenced public databases, including ACMG 73, ClinVar, NHGRI-GWAS catalog, CPIC, PharmGKB, and PharmaADME, and can be directly genotyped for each sample.

The Axiom PangenomiX Array also offers CNV analysis for fixed genomic regions and de novo copy number discovery to detect copy number changes across the whole genome.

Scientists requiring genotyping of important pharmacogenes in difficult to genotype regions, such as CYP2D6, the 'Plus' version of the array, Axiom™ PangenomiX Plus Array should be ordered. Axiom PangenomiX Plus Array also comes with Pharmacogenomic Translation Reports with star alleles and general metabolizer status.

Content Coverage:
• Genome-wide association study (GWAS) imputation module with ∼850,000 markers across all ancestral populations
• Evidence-based markers for relevant variants including ClinVar variants for comprehensive coverage of ACMG73 genes
• PGx variants cited in Clinical Pharmacogenomics Implementation Consortium (CPIC®) guidelines and Pharmacogenomics Knowledge Base (PharmGKB®)
• Disease-related variants (Alzheimer's, cancer, cardiovascular disease, cardiometabolic disease, diabetes, neurological disorders)
• Blood phenotyping variants for blood typing of common and rare blood groups, bleeding disorders, and blood conditions such as sickle cell anemia
• Human Leukocyte Antigen (HLA) type of 11 major MHC (major histocompatibility complex) Class I and Class II loci
• SARS-CoV-2 immune-related markers (human leukocyte antigens, killer immunoglobulin-like receptors, autoimmune markers, and inflammatory markers)
• Functional variants (loss of function variants, expression quantitative trait loci, nonsynonymous variants, lung phenotypes)
• Ancestry, wellness, and trait-related variants

Samples can be processed on the 96-format Axiom PangenomiX Array using the Axiom 2.0 manual workflow and the Axiom 2.0 Propel Fast Wash Workflow, to support low to high throughput users. Final array processing is done on the GeneTitan MC Fast Scan Instrument. The Axiom PangenomiX Array is sold as a kit and includes the array, Axiom 2.0 reagents and GeneTitan MC Fast Scan consumables.

The Axiom PangenomiX Array genotype analysis is supported by Axiom Analysis Suite Software. Axiom HLA Analysis Software can also be used for HLA typing.

For those looking for deeper scientific insights or to include specific SNPs relevant to their disease research area or population-specific variants of interest, Axiom myDesign Custom Array Services are available for the creation of a custom array.

For researchers interested in getting data quickly in absence of your own dedicated wet-lab, contact our global Microarray Research Services Laboratory to genotype your samples on the Axiom PangenomiX Array and receive results ready for immediate downstream analysis.

CPIC® is a registered service mark of the US Department of Health & Human Services (HHS).

PharmGKB® is a registered trademark of HHS.