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Amino Allyl MessageAmp™ II aRNA 扩增试剂盒
The Amino Allyl II MessageAmp™ aRNA Amplification Kit includes reagents for aRNA amplification with amimo allyl NTP incorporation. The kit了解更多信息
货号 AM1753
价格（CNY)/ EA 20 reactions
36,395.00
-
价格（CNY)/ EA 20 reactions
36,395.00
Amino Allyl MessageAmp™ II aRNA 扩增试剂盒
货号AM1753
价格（CNY)/ EA 20 reactions
36,395.00
-
The Amino Allyl II MessageAmp™ aRNA Amplification Kit includes reagents for aRNA amplification with amimo allyl NTP incorporation. The kit includes sufficient reagents for 20 reactions. Cy™ dyes are not included.
Improved first- and second-strand cDNA synthesis
Included in the kit is ArrayScript™ RT, a rationally engineered M-MLV reverse transcriptase, which produces equivalent or higher yields of full-length cDNA compared to other enzymes. The second-strand cDNA synthesis reaction has also been optimized to be compatible with the first-strand cDNA products generated with ArrayScript™ RT to allow maximal conversion of first-strand cDNA into full-length double-stranded cDNA templates.
Increased labeling efficiency of your aRNA
The Amino Allyl MessageAmp™ II aRNA Amplification Kit incorporates amino allyl NTP into the aRNA followed by the coupling of its reactive amino group to an NHS ester label (e.g., Cy™ or another dye). This strategy offers several advantages over the direct incorporation of labeled NTPs. Direct incorporation of labeled NTPs is inefficient and results in low yields and low specific activity aRNA and high costs. Unlike lableled NTPs, amino allyl–modified NTPs are incorporated almost as efficiently as unmodified NTPs and are much less expensive than the dye coupled NTPs.
Improved first- and second-strand cDNA synthesis
Included in the kit is ArrayScript™ RT, a rationally engineered M-MLV reverse transcriptase, which produces equivalent or higher yields of full-length cDNA compared to other enzymes. The second-strand cDNA synthesis reaction has also been optimized to be compatible with the first-strand cDNA products generated with ArrayScript™ RT to allow maximal conversion of first-strand cDNA into full-length double-stranded cDNA templates.
Increased labeling efficiency of your aRNA
The Amino Allyl MessageAmp™ II aRNA Amplification Kit incorporates amino allyl NTP into the aRNA followed by the coupling of its reactive amino group to an NHS ester label (e.g., Cy™ or another dye). This strategy offers several advantages over the direct incorporation of labeled NTPs. Direct incorporation of labeled NTPs is inefficient and results in low yields and low specific activity aRNA and high costs. Unlike lableled NTPs, amino allyl–modified NTPs are incorporated almost as efficiently as unmodified NTPs and are much less expensive than the dye coupled NTPs.
仅供科研使用。不可用于诊断程序。
规格
产品类型aRNA 扩增试剂盒
包括标签或染料否
标记方法间接标记
逆转录酶ArrayScript™
样品类型poly(A+) RNA、总 RNA
产品规格试剂盒
产品线Ambion™、MessageAmp™
数量20 次反应
Unit SizeEA 20 reactions
内容与储存
• 60 µl T7 Oligo(dT) 引物 (-20°C)
• 22 µL ArrayScript™ 逆转录酶 (-20°C)
• 22 µL RNase 抑制剂 (-20°C)
• 42 µL 10X 第一链缓冲液 (-20°C)
• 170 µL dNTP 混合物 (-20°C)
• 210 µL 10X 第二链缓冲液 (-20°C)
• 42 µL DNA 聚合酶 (-20°C)
• 22 µL RNase H (-20°C)
• 84 µL T7 酶混合物 (-20°C)
• 84 µL 10X 反应缓冲液 (-20°C)
• 95 µL 50 mM UTP 溶液 (-20°C)
• 64 µL 50 mM 5-（3-氨基烯丙基）-UTP (-20°C)
• 255 µL ATP、CTP、GTP 混合物 (-20°C)
• 40 µl 第二轮引物 (-20°C)
• 10 µl 1 mg/mL 对照 RNA (-20°C)
• 1.75 ml 无核酸酶水（任何温度）
• 400 µl 偶联缓冲液 (-20°C)
• 440 µl DMSO (-20°C)
• 180 µl 4M 羟胺 (-20°C)
• 40 ml 洗涤缓冲液（4°C 或室温）
• 7 ml cDNA 结合缓冲液（室温）
• 20 ml aRNA 结合缓冲液（室温）
• 20 aRNA 滤芯（室温）
• 40 aRNA 收集管（室温）
• 20 cDNA 过滤柱芯 + 管（室温）
• 20 cDNA 洗脱管（室温）
• 10 ml 无核酸酶水（任何温度）
• 20 个标记 aRNA 滤芯 + 管（室温）
• 20 个标记 aRNA 洗脱管（室温）
• 22 µL ArrayScript™ 逆转录酶 (-20°C)
• 22 µL RNase 抑制剂 (-20°C)
• 42 µL 10X 第一链缓冲液 (-20°C)
• 170 µL dNTP 混合物 (-20°C)
• 210 µL 10X 第二链缓冲液 (-20°C)
• 42 µL DNA 聚合酶 (-20°C)
• 22 µL RNase H (-20°C)
• 84 µL T7 酶混合物 (-20°C)
• 84 µL 10X 反应缓冲液 (-20°C)
• 95 µL 50 mM UTP 溶液 (-20°C)
• 64 µL 50 mM 5-（3-氨基烯丙基）-UTP (-20°C)
• 255 µL ATP、CTP、GTP 混合物 (-20°C)
• 40 µl 第二轮引物 (-20°C)
• 10 µl 1 mg/mL 对照 RNA (-20°C)
• 1.75 ml 无核酸酶水（任何温度）
• 400 µl 偶联缓冲液 (-20°C)
• 440 µl DMSO (-20°C)
• 180 µl 4M 羟胺 (-20°C)
• 40 ml 洗涤缓冲液（4°C 或室温）
• 7 ml cDNA 结合缓冲液（室温）
• 20 ml aRNA 结合缓冲液（室温）
• 20 aRNA 滤芯（室温）
• 40 aRNA 收集管（室温）
• 20 cDNA 过滤柱芯 + 管（室温）
• 20 cDNA 洗脱管（室温）
• 10 ml 无核酸酶水（任何温度）
• 20 个标记 aRNA 滤芯 + 管（室温）
• 20 个标记 aRNA 洗脱管（室温）