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产品规格
检测灵敏度
<10 pg/mL
测定范围
15.6-1000 pg/mL
检测样本类型/体积
{Cerebrospinal Fluid=50 µL, Supernatant=50 µL, Tissue Homogenate=50 µL}
手工操作时间
1 hr 20 min
总时间
4 hr
均相（无需洗涤）
No
批间差异
4.3%
批内差异
3.6%
检测仪器
Colorimetric Microplate Reader
产品规格
2 x 96 Tests
产品成分
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Standard Dilution Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
储存
2-8°C
蛋白名称
Amyloid beta 42
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Amyloid Beta 42 (Hu Abeta 42) ELISA quantitates Hu Abeta 42 in human tissue culture medium, tissue homogenate, and cerebrospinal fluid (CSF). The assay will exclusively recognize both natural and recombinant Hu Abeta 42.
原理方法
The Human Abeta 42 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Beta amyloid is a 40-43 amino acid peptide cleaved from amyloid precursor protein by a protease, gamma-secretase. Beta Amyloid (1-40) (Amyloid beta 40 or Abeta 40) together with beta Amyloid (1-42) (Amyloid beta 42 or Abeta 42) are two major C-terminal variants of the amyloid beta protein, constituting the majority of amyloid beta proteins. Both the Amyloid beta 40 and Amyloid beta 42 alloforms are major components of the extracellular plaques found in Alzheimer's disease brain tissue.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : A4, AAA, ABETA, ABPP, AD1, APP, APPI, CTFgamma, CVAP, PN-II, PN2
基因ID : (Human) 351
基因符号 : APP
蛋白别名 : Abeta40, Abeta42, ABPP, AICD-50, AICD-57, AICD-59, Alpha-CTF, Alpha-secretase C-terminal fragment, Alzheimer disease amyloid A4 protein homolog, Alzheimer disease amyloid protein, amyloid beta (A4) precursor protein, Amyloid intracellular domain 50, Amyloid intracellular domain 57, Amyloid intracellular domain 59, Amyloid precursor protein, Amyloid-beta (A4) precursor protein, Amyloid-beta A4 protein, Amyloid-beta precursor protein, Amyloid-beta protein 40, Amyloid-beta protein 42, APP, APPI, BAPP42, BetaAPP42, β APP42, βAPP42, AB42, Amyloid b, Amyloid beta, Amyloid beta 1 42, Amyloid β, Aβ 1 42, B APP42, A beta 1 42, A β 1 42, Amyloid, beta-amyloid peptide, beta-amyloid peptide(1-40), beta-amyloid peptide(1-42), beta-amyloid precursor protein, Beta-APP40, Beta-APP42, Beta-CTF, Beta-secretase C-terminal fragment, C31, C80, C83, C99, Cerebral vascular amyloid peptide, CVAP, Gamma-CTF(50), Gamma-CTF(57), Gamma-CTF(59), Gamma-secretase C-terminal fragment 50, Gamma-secretase C-terminal fragment 57, Gamma-secretase C-terminal fragment 59, N-APP, P3(40), P3(42), peptidase nexin-II, PN-II, PreA4, Protease nexin-II, S-APP-alpha, S-APP-beta, Soluble APP-alpha, Soluble APP-beta, testicular tissue protein Li 2
UniProt ID (Human) Q9UQ58
