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产品规格
检测灵敏度
0.5 ng/mL
测定范围
0.94-60 ng/mL
检测样本类型/体积
{Cell Lysate=10 µL}
手工操作时间
1 hr 20 min
总时间
4 hr
均相（无需洗涤）
No
批间差异
8.2%
批内差异
5.4%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
INSR beta
蛋白亚型
Receptor Tyrosine Kinases
蛋白家族
Kinases & Inhibitors
均相（无需洗涤）
Human, Mouse, Rat
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human Insulin Receptor beta subunit (Hu IRβ) ELISA quantitates Hu IRβ in human cell and tissue lysates. The assay will exclusively recognize both natural and recombinant Hu IRβ. Cross-reactivity has been observed in mouse and rat cells.
原理方法
The Human IRβ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Biological actions of insulin and IGF-1 are mediated by their respective cell surface receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades. The insulin receptor. Insulin/IGF-1 binding to the extracellular domain leads to autophosphorylation of downstream target proteins. These two receptors differ in sequence in regions that confer specificity for the designated ligand as well as in certain intracellular signaling domains, resulting in significant differences in the functional consequences of activation of each receptor. Defects in IR are the cause of various insulin resistance syndromes and IGF-1R defects may cause some forms of growth retardation. Both these signaling cascades are also important for the development of cancer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : 4932439J01Rik, CD220, D630014A15Rik, HHF5, INSR, IR, IR-A, IR-B
基因ID : (Human) 3643, (Rat) 24954, (Mouse) 16337
基因符号 : INSR, Insr, Insr
蛋白别名 : CD220, Insulin receptor, insulin receptor preproprotein, Insulin receptor subunit alpha, Insulin receptor subunit beta, IR, CD220 beta, CD221, IGF-I receptor, IR beta
UniProt ID (Human) P06213, (Mouse) P15208
