Invitrogen

OCT4 Monoclonal Antibody (9B7)

OCT4 Antibody in Immunocytochemistry (ICC/IF)

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OCT4 Antibody (MA1-104) in ICC/IF

Immunofluorescence analysis of Oct4/Pou5F1 was done on 70% confluent log phase NTREA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Oct4/Pou5F1 (9B7) Mouse Monoclonal Antibody (Product # MA1-104) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More

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OCT4 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Expression of Oct4 was observed specifically in embryonic carcinoma cell lines such as NCCIT, NTERA2 and F9 using Oct4 Mouse Monoclonal Antibody (Product # MA1-104) in western blot. {RE}

产品信息

MA1-104

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:5,000

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免疫组化（石蜡） (IHC (P))

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免疫细胞化学 (ICC/IF)

1-2 µg/mL

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流式细胞分析 (Flow)

3-5 µg/1x10^6 cells

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产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

9B7

抗原

Full length human recombinant protein of human POU5F1 produced in E.coli.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2536771

产品详细信息

MA1-104 detects Oct4/Pou5F1 in Human embryonic carcinomic samples, but not in adult samples (e.g. HeLa cells). This antibody did not show reactivity in a mouse embryonic cell line (i.e. F9 embryonic carcinoma cells).

MA1-104 has been successfully used in FACS, IF, and WB. By Western blot, this antibody detects a band at 40 kDa in human NCCIT and NTERA-2 embryonal carcinoma cells, but not in negative control cells.

靶标信息

POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Comparison of human recombinant protein coatings and fibroblast-ECM to Matrigel for induced pluripotent stem cell culture and renal podocyte differentiation.

ALTEX

Murphy C,Naderlinger E,Mater A,Kluin RJC,Wilmes A

MA1-104 was used in Immunocytochemistry-immunoflourescence to show that all tested coatings were highly comparable to the animal-derived Matrigel for both hiPSC maintenance and differentiation into renal podocyte-like cells.

Thu Jan 26 00:00:00 UTC 2023

Hypoxia enhances anti-fibrotic properties of extracellular vesicles derived from hiPSCs via the miR302b-3p/TGFβ/SMAD2 axis.

BMC medicine

Paw M,Kusiak AA,Nit K,Litewka JJ,Piejko M,Wnuk D,Sarna M,Fic K,Stopa KB,Hammad R,Barczyk-Woznicka O,Cathomen T,Zuba-Surma E,Madeja Z,Ferdek PE,Bobis-Wozowicz S

MA1-104 was used in Immunocytochemistry to provide evidence of superior anti-fibrotic properties of EV-H5 over EV-N or EV-H3.

Tue Oct 31 00:00:00 UTC 2023

Hypoxia enhances anti-fibrotic properties of extracellular vesicles derived from hiPSCs via the miR302b-3p/TGFβ/SMAD2 axis.

BMC medicine

Paw M,Kusiak AA,Nit K,Litewka JJ,Piejko M,Wnuk D,Sarna M,Fic K,Stopa KB,Hammad R,Barczyk-Woznicka O,Cathomen T,Zuba-Surma E,Madeja Z,Ferdek PE,Bobis-Wozowicz S

MA1-104 was used in Immunocytochemistry to provide evidence of superior anti-fibrotic properties of EV-H5 over EV-N or EV-H3.

Tue Oct 31 00:00:00 UTC 2023

Breast cancer stem cells generate immune-suppressive T regulatory cells by secreting TGFβ to evade immune-elimination.

Discover oncology

Mukherjee S,Chakraborty S,Basak U,Pati S,Dutta A,Dutta S,Roy D,Banerjee S,Ray A,Sa G,Das T

MA1-104 was used in Immunohistochemistry (Paraffin) to show that during initiation as well as after chemotherapy, when NSCCs are not present in the tumor microenvironment, CSCs, albeit present in low numbers, generate immunosuppressive CD4+CD25+FOXP3+ Treg cells in a contact-independent manner by shedding high levels of immune-suppressive Treg-polarizing cytokine TGFβ, thus escaping immune-elimination and initiating the tumor or causing tumor relapse.

Fri Dec 01 00:00:00 UTC 2023

Fine-tuned KDM1A alternative splicing regulates human cardiomyogenesis through an enzymatic-independent mechanism.

iScience

Astro V,Ramirez-Calderon G,Pennucci R,Caroli J,Saera-Vila A,Cardona-Londoño K,Forastieri C,Fiacco E,Maksoud F,Alowaysi M,Sogne E,Falqui A,Gonzàlez F,Montserrat N,Battaglioli E,Mattevi A,Adamo A

MA1-104 was used in Flow cytometry/Cell sorting to prove the existence of a divergent scaffolding role of KDM1A splice variants, independent of their enzymatic activity, during hESC differentiation into cardiac cells.

Fri Jul 15 00:00:00 UTC 2022

Establishment of an iPSC cohort from three unrelated 47-XXY Klinefelter Syndrome patients (KAUSTi007-A, KAUSTi007-B, KAUSTi009-A, KAUSTi009-B, KAUSTi010-A, KAUSTi010-B).

Stem cell research

Alowaysi M,Fiacco E,Astro V,Adamo A

MA1-104 was used in Flow Cytometry to describe the derivation of multiple 47-XXY iPSC lines from three unrelated KS patients to study the impact of supernumerary X chromosome during early development.

Tue Dec 01 00:00:00 UTC 2020

Establishment of an iPSC cohort from three unrelated 47-XXY Klinefelter Syndrome patients (KAUSTi007-A, KAUSTi007-B, KAUSTi009-A, KAUSTi009-B, KAUSTi010-A, KAUSTi010-B).

Stem cell research

Alowaysi M,Fiacco E,Astro V,Adamo A

Tue Dec 01 00:00:00 UTC 2020

Derivation of two naturally isogenic iPSC lines (KAUSTi006-A and KAUSTi006-B) from a mosaic Klinefelter Syndrome patient (47-XXY/46-XY).

Stem cell research

Fiacco E,Alowaysi M,Astro V,Adamo A

MA1-104 was used in Flow cytometry/Cell sorting to show that using naturally isogenic iPSCs from mosaic Klinefelter Syndrome patients to compare disease and healthy cells carrying a virtually identical genomic background.

Tue Dec 01 00:00:00 UTC 2020

Generation of two iPSC lines (KAUSTi001-A, KAUSTi002-A) from a rare high-grade Klinefelter Syndrome patient (49-XXXXY) carrying a balanced translocation t(4,11) (q35,q23).

Stem cell research

Alowaysi M,Fiacco E,Astro V,Adamo A

MA1-104 was used in Immunocytochemistry to generate two iPSC lines 48-XXXY and 49-XXXXY from a non-mosaic 49-XXXXY KS patient carrying a balanced translocation t(4,11) (q35,q23).

Tue Dec 01 00:00:00 UTC 2020

Establishment of iPSC lines from a high-grade Klinefelter Syndrome patient (49-XXXXY) and two genetically matched healthy relatives (KAUSTi003-A, KAUSTi004-A, KAUSTi004-B, KAUSTi005-A, KAUSTi005-B, KAUSTi005-C).

Stem cell research

Alowaysi M,Fiacco E,Astro V,Adamo A

MA1-104 was used in Immunocytochemistry to derived iPSC lines from a high-grade 49-XXXXY Klinefelter Syndrome and two healthy donors' fibroblasts.

Tue Dec 01 00:00:00 UTC 2020

Generation of iPSC lines (KAUSTi011-A, KAUSTi011-B) from a Saudi patient with epileptic encephalopathy carrying homozygous mutation in the GLP1R gene.

Stem cell research

Alowaysi M,Astro V,Fiacco E,Alzahrani F,Alkuraya FS,Adamo A

MA1-104 was used in Immunocytochemistry to establish two iPSC lines from a patient harbouring a rare homozygous splice site variant in GLP1R for use as a model to study the molecular pathology of GLP1R dysfunction.

Mon Dec 28 00:00:00 UTC 2020

Applying hydrodynamic pressure to efficiently generate induced pluripotent stem cells via reprogramming of centenarian skin fibroblasts.

PloS one

Vosough M,Ravaioli F,Zabulica M,Capri M,Garagnani P,Franceschi C,Piccand J,Kraus MR,Kannisto K,Gramignoli R,Strom SC

MA1-104 was used in Immunocytochemistry to show efficient generation of iPSCs from the elderly may provide a source of cells for the regeneration of tissues and organs with autologous cells as well as cellular models for the study of aging, longevity and age-related diseases.

Fri Feb 14 00:00:00 UTC 2020

Hollow microcarriers for large-scale expansion of anchorage-dependent cells in a stirred bioreactor.

Biotechnology and bioengineering

YekrangSafakar A,Acun A,Choi JW,Song E,Zorlutuna P,Park K

MA1-104 was used in Immunocytochemistry-immunofluorescence to demonstrate the expansion of fibroblasts, NIH/3T3 and the expansion and cardiac differentiation of human induced pluripotent stem cells, along with detailed numerical analysis.

Sun Jul 01 00:00:00 UTC 2018

Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation.

Stem cell reports

Balboa D,Weltner J,Eurola S,Trokovic R,Wartiovaara K,Otonkoski T

MA1-104 was used in immunocytochemistry to demonstrate that dCas9 activator controls human pluripotent stem cell differentiation into endodermal lineages

Tue Sep 08 00:00:00 UTC 2015

生物信息学

蛋白别名: 3-Oct; 4-Oct; DADB-104B20.2; gp-9; HGNC:9221; MGC22487; NF-A3; Oct-3; Oct-4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF-3; OTTHUMP00000221150; OTTHUMP00000221151; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; pou-2; POU-type homeodomain-containing DNA-binding protein; pou2

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基因别名: NF-A3; Oct-3; Oct-3/4; Oct-4; OCT3; Oct3/4; OCT4; OTF-3; Otf-4; OTF3; Otf3-rs7; Otf3g; OTF4; POU5F1

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UniProt ID: (Human) Q01860, (Mouse) P20263

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Entrez Gene ID: (Human) 5460, (Mouse) 18999

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功能