Invitrogen

SOX2 Monoclonal Antibody (10F10C9)

SOX2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

SOX2 Antibody (MA5-15449) in ICC/IF

Immunofluorescence analysis of SOX2 was performed using 70% confluent log phase NTERA-2 cl.D1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SOX2 Monoclonal Antibody (10F10C9) (Product # MA5-15449) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488... View More

Product Image Gallery

SOX2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of SOX2 was observed in HEK-293 and NTERA-2 in comparison to HeLa and THP-1 using Anti-SOX2 Monoclonal Antibody (10F10C9) (Product # MA5-15449) in Western Blot. {RE}

产品信息

MA5-15449

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫细胞化学 (ICC/IF)

1:100

酶联免疫吸附实验 (ELISA)

1:10,000

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

10F10C9

抗原

Purified recombinant fragment of SOX2 (aa1-170) expressed in E. Coli.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

ascites

内含物

0.03% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_10976833

产品详细信息

MA5-15449 targets SOX2 in indirect ELISA, WB applications and shows reactivity with Human samples.

The MA5-15449 immunogen is purified recombinant fragment of SOX2 (aa1-170) expressed in E. Coli.

MA5-15449 detects SOX2 which has a predicted molecular weight of approximately 34.3kDa.

靶标信息

SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3); sex determining region Y-box 2; SOX 2; SRY (sex determining region Y) box 2; SRY (sex determining region Y)-box 2; SRY box 2; SRY related HMG box 2; SRY-related HMG-box gene 2; Transcription factor SOX-2; transcription factor SOX2

查看更多收起

基因别名: ANOP3; MCOPS3; SOX2

查看更多收起

UniProt ID: (Human) P48431

查看更多收起

Entrez Gene ID: (Human) 6657

查看更多收起

功能

transcription regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding miRNA binding sequence-specific DNA binding transcription regulatory region DNA binding

参与通路