Invitrogen

OTX2 Monoclonal Antibody (1H12C4B5)

OTX2 Antibody in Immunocytochemistry (ICC/IF)

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OTX2 Antibody (MA5-15854) in ICC/IF

Immunofluorescence analysis of HepG2 cells using OTX2 monoclonal antibody (Product # MA5-15854) (Green). Red: actin filaments have been labeled with phalloidin.

OTX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

OTX2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

OTX2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of OTX2 was observed in Mouse retina in comparison to Mouse Brain, Mouse Eye and Mouse Lung using Anti-OOTX2 Monoclonal Antibody (1H12C4B5) (Product # MA5-15854) in Western Blot. {RE}

产品信息

MA5-15854

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化 (IHC)

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

免疫细胞化学 (ICC/IF)

1:200-1:1,000

流式细胞分析 (Flow)

1:200-1:400

酶联免疫吸附实验 (ELISA)

1:10,000

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

1H12C4B5

抗原

Purified recombinant fragment of human OTX2 expressed in E. Coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

ascites

内含物

0.03% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11155193

产品详细信息

MA5-15854 targets OTX2 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15854 immunogen is purified recombinant fragment of human OTX2 expressed in E. Coli. .

MA5-15854 detects OTX2 which has a predicted molecular weight of approximately 32kDa.

靶标信息

OTX2 is a 289 amino acid containing protein encoding a member of the bicoid sub-family of homeodomain-containing transcription factors. It has two known alternative splice variants with distinct isoforms though presence of other isoforms cannot be excluded. The protein is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sensory organs. The protein may bind to BCD target sequence (BTS): 5-'TCTAATCCC-3'. Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Constitutive activation of canonical Wnt signaling disrupts choroid plexus epithelial fate.

Nature communications

Parichha A,Suresh V,Chatterjee M,Kshirsagar A,Ben-Reuven L,Olender T,Taketo MM,Radosevic V,Bobic-Rasonja M,Trnski S,Holtzman MJ,Jovanov-Milosevic N,Reiner O,Tole S

MA5-15854 was used in Immunohistochemistry to study the presence of molecules downstream of cannonical Wnt signaling in vivo at embryonic stages in the human and mouse choroid plexus.

Wed Feb 02 00:00:00 UTC 2022

Constitutive activation of canonical Wnt signaling disrupts choroid plexus epithelial fate.

Nature communications

Parichha A,Suresh V,Chatterjee M,Kshirsagar A,Ben-Reuven L,Olender T,Taketo MM,Radosevic V,Bobic-Rasonja M,Trnski S,Holtzman MJ,Jovanov-Milosevic N,Reiner O,Tole S

MA5-15854 was used in Immunohistochemistry to study the presence of molecules downstream of cannonical Wnt signaling in vivo at embryonic stages in the human and mouse choroid plexus.

Wed Feb 02 00:00:00 UTC 2022

Generation of human induced pluripotent stem cell-derived cerebral organoids for cellular and molecular characterization.

STAR protocols

Anastasaki C,Wilson AF,Chen AS,Wegscheid ML,Gutmann DH

Published figure using OTX2 monoclonal antibody (Product # MA5-15854) in Immunohistochemistry

Fri Mar 18 00:00:00 UTC 2022

Generation of human induced pluripotent stem cell-derived cerebral organoids for cellular and molecular characterization.

STAR protocols

Anastasaki C,Wilson AF,Chen AS,Wegscheid ML,Gutmann DH

Published figure using OTX2 monoclonal antibody (Product # MA5-15854) in Immunohistochemistry

Fri Mar 18 00:00:00 UTC 2022

Co-activation of Sonic hedgehog and Wnt signaling in murine retinal precursor cells drives ocular lesions with features of intraocular medulloepithelioma.

Oncogenesis

Dottermusch M,Sumisławski P,Krevet J,Middelkamp M,Voß H,Siebels B,Bartsch H,Sotlar K,Meyer P,Frank S,Korshunov A,Glatzel M,Schüller U,Neumann JE

Published figure using OTX2 monoclonal antibody (Product # MA5-15854) in Immunohistochemistry

Tue Nov 16 00:00:00 UTC 2021

Patient-derived iPSC-cerebral organoid modeling of the 17q11.2 microdeletion syndrome establishes CRLF3 as a critical regulator of neurogenesis.

Cell reports

Wegscheid ML,Anastasaki C,Hartigan KA,Cobb OM,Papke JB,Traber JN,Morris SM,Gutmann DH

MA5-15854 was used in Immunohistochemistry to identify both neural stem cell (NSC) proliferation and neuronal maturation abnormalities in NF1-TGD hCOs.

Tue Jul 06 00:00:00 UTC 2021

Retinal inputs signal astrocytes to recruit interneurons into visual thalamus.

Proceedings of the National Academy of Sciences of the United States of America

Su J,Charalambakis NE,Sabbagh U,Somaiya RD,Monavarfeshani A,Guido W,Fox MA

MA5-15854 was used in Immunohistochemistry to show that retinal inputs signal through astrocytes to direct the long-range recruitment of interneurons into the visual thalamus.