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PDGFRA Antibody (PA5-16571) in ICC/IF
Immunofluorescence analysis of PDGFR alpha was performed using 70% confluent log phase NIH/3T3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PDGFR alpha Rabbit Polyclonal Antibody (Product # PA5-16571) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµga... View More
产品信息
PA5-16571
应用
建议稀释比
已发表文章
产品规格
已发表种属
Human,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide derived from the C-terminus of the precursor form of human PDGFR, alpha
偶联物
形式
Liquid
浓度
1 mg/mL
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10981626
产品详细信息
PA5-16571 targets PDGFR alpha in IHC applications and shows reactivity with mouse, Rat, and Human samples.
The PA5-16571 immunogen is a synthetic peptide derived from the C-terminus of the precursor form of human PDGFR, alpha.
靶标信息
PDGFRA is a cell surface receptor tyrosine kinase which binds members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
