Invitrogen

c-Kit Polyclonal Antibody

c-Kit Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 1

c-Kit Antibody (PA5-16770) in IHC (P)

Formalin-fixed, paraffin-embedded human stroma tumor stained with CD117/c-kit antibody using peroxidase-conjugate and AEC. Note staining of tumor cells.

Product Image Gallery

产品信息

PA5-16770

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100

查看2篇已发表文章 2篇已发表文章

其他PubMed (MISC)

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse, Pig, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide from the cytoplasmic domain of human CD117 / c-kit protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Affinity chromatography

保存液

PBS, pH 7.6, with 1% BSA

内含物

0.1% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_10982522

产品详细信息

PA5-16770 targets CD117 in IHC (P) applications and shows reactivity with Canine, Rat and Human samples.

The PA5-16770 immunogen is a synthetic peptide from the cytoplasmic domain of human CD117 / c-kit protein.

靶标信息

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Cardiac telocytes inhibit cardiac microvascular endothelial cell apoptosis through exosomal miRNA-21-5p-targeted cdip1 silencing to improve angiogenesis following myocardial infarction.

Theranostics

Liao Z,Chen Y,Duan C,Zhu K,Huang R,Zhao H,Hintze M,Pu Q,Yuan Z,Lv L,Chen H,Lai B,Feng S,Qi X,Cai D

PA5-16770 was used in Magnetic cell separation to study whether cardiac telocyte-endothelial cell communication suppresses apoptosis and promotes the survival of endothelial cells to facilitate cardiac angiogenesis during myocardial infarction.

Thu Aug 05 00:00:00 UTC 2021

Mast Cells in the Auditory Periphery of Rodents.

Brain sciences

Szczepek AJ,Dudnik T,Karayay B,Sergeeva V,Olze H,Smorodchenko A

PA5-16770 was used in Western Blot, Immunohistochemistry (Paraffin) to show that MCs are present in the cochleae of C57BL/6 mice and Wistar rats, where they localize in the modiolus, spiral ligament, and stria vascularis.

Thu Oct 01 00:00:00 UTC 2020

Mast Cells in the Auditory Periphery of Rodents.

Brain sciences

Szczepek AJ,Dudnik T,Karayay B,Sergeeva V,Olze H,Smorodchenko A

Thu Oct 01 00:00:00 UTC 2020

Genetic diversity between mouse strains allows identification of the CC027/GeniUnc strain as an orally reactive model of peanut allergy.

The Journal of allergy and clinical immunology

Orgel K,Smeekens JM,Ye P,Fotsch L,Guo R,Miller DR,Pardo-Manuel de Villena F,Burks AW,Ferris MT,Kulis MD

PA5-16770 was used in Immunohistochemistry to assess food allergy phenotypes in a genetically diverse collection of mice.

Fri Mar 01 00:00:00 UTC 2019

Changes in the Neuronal Control of the Urinary Bladder in a Model of Radiation Cystitis.

The Journal of pharmacology and experimental therapeutics

Giglio D,Podmolíková L,Tobin G

PA5-16770 was used in Immunohistochemistry to propose bladder irradiation affects several levels of neuronal control of the urinary bladder.

Tue May 01 00:00:00 UTC 2018

SDF1 gradient associates with the distribution of c-Kit+ cardiac cells in the heart.

Scientific reports

Renko O,Tolonen AM,Rysä J,Magga J,Mustonen E,Ruskoaho H,Serpi R

PA5-16770 was used in Immunohistochemistry to investigate molecular targets to be utilised in modulating the mobility of endogenous cardiac stem cells.

Thu Jan 18 00:00:00 UTC 2018

Phase 2 trial of dasatinib in target-selected patients with recurrent glioblastoma (RTOG 0627).

Neuro-oncology

Lassman AB,Pugh SL,Gilbert MR,Aldape KD,Geinoz S,Beumer JH,Christner SM,Komaki R,DeAngelis LM,Gaur R,Youssef E,Wagner H,Won M,Mehta MP

PA5-16770 was used in immunohistochemistry - paraffin section to run phase 2 trial of dasatinib in target-selected patients with recurrent glioblastoma

Wed Jul 01 00:00:00 UTC 2015

Wharton's jelly-derived mesenchymal stem cells promote myocardial regeneration and cardiac repair after miniswine acute myocardial infarction.

Coronary artery disease

Zhang W,Liu XC,Yang L,Zhu DL,Zhang YD,Chen Y,Zhang HY

PA5-16770 was used in immunohistochemistry to use a miniswine model of acute myocardial infarction to study the therapeutic effects of Wharton's jelly-derived mesenchymal stem cells on cardiac repair and performance

Fri Nov 01 00:00:00 UTC 2013

生物信息学

蛋白别名: CD117; ckit; Mast/stem cell growth factor receptor Kit; p145 c-kit; PBT; Piebald trait protein; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

查看更多收起

基因别名: C-Kit; CD117; KIT; PBT; SCFR

查看更多收起

UniProt ID: (Human) P10721

查看更多收起

Entrez Gene ID: (Human) 3815

查看更多收起

功能