Invitrogen

CD3 Monoclonal Antibody (SK7), APC, eBioscience™

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CD3 Antibody (17-0036-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD3 APC (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD3 Antibody — Staining of human peripheral blood cells. As expected based on known relative expression patterns, CD3 clone SK7 stains a subset of lymphocytes (T cells) and does not stain monocytes and granulocytes (middle plot). Additional analysis of lymphocytes shows that CD3 clone SK7 does not stain any CD19+ B cells (right plot). Details: Normal human whole blood was surface stained with CD3 (clone SK7) and CD19 (clone HIB19). After staining, red blood cells were lysed using 1-step Fix/Lyse Buffer. Cells in the lymphocyte (purple histogram), monocyte (orange histogram), or granulocyte (blue histogram) gates were used to compare CD3 staining (middle plot). Cells in the lymphocyte gate were used to compare CD3 and CD19 staining (right plot). {RE}

产品信息

17-0036-42

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

查看18篇已发表文章 18篇已发表文章

产品规格

种属反应

Chimpanzee, Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

SK7

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10596664

产品详细信息

Description: The SK7 monoclonal antibody reacts with human and chimpanzee CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. The SK7 and UCHT1 monoclonal antibodies cross-block binding, suggesting recognition of overlapping epitope. In contrast, clones OKT3 and SK7 see different epitopes.

The antibody SK7 recognizes chimpanzee CD3.

Applications Reported: This SK7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SK7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Targeting myeloid-derived suppressor cells with gemcitabine to enhance efficacy of adoptive cell therapy in bladder cancer.

Frontiers in immunology

Bazargan S,Bunch B,Ojwang' AME,Blauvelt J,Landin A,Ali J,Abrahams D,Cox C,Hall AM,Beatty MS,Poch M,Rejniak KA,Pilon-Thomas S

17-0036-42 was used in Flow Cytometry to show that Gemcitabine can be used to lymphodeplete bladder tumors and precondition the microenvironment for intravesical ACT.

Tue Oct 31 00:00:00 UTC 2023

Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer.

The Journal of biological chemistry

Cha JH,Chan LC,Wang YN,Chu YY,Wang CH,Lee HH,Xia W,Shyu WC,Liu SP,Yao J,Chang CW,Cheng FR,Liu J,Lim SO,Hsu JL,Yang WH,Hortobagyi GN,Lin C,Yang L,Yu D,Jeng LB,Hung MC

Published figure using CD3 monoclonal antibody (Product # 17-0036-42) in Flow Cytometry

Fri Apr 01 00:00:00 UTC 2022

Direct and indirect immune effects of CMP-001, a virus-like particle containing a TLR9 agonist.

Journal for immunotherapy of cancer

Sabree SA,Voigt AP,Blackwell SE,Vishwakarma A,Chimenti MS,Salem AK,Weiner GJ

17-0036-42 was used in Flow Cytometry to explore mechanisms by which the initial effects of CMP-001 on pDCs activate other cells that can contribute to development of an antitumor T-cell response.

Tue Jun 01 00:00:00 UTC 2021

Direct and indirect immune effects of CMP-001, a virus-like particle containing a TLR9 agonist.

Journal for immunotherapy of cancer

Sabree SA,Voigt AP,Blackwell SE,Vishwakarma A,Chimenti MS,Salem AK,Weiner GJ

17-0036-42 was used in Flow Cytometry to explore mechanisms by which the initial effects of CMP-001 on pDCs activate other cells that can contribute to development of an antitumor T-cell response.

Tue Jun 01 00:00:00 UTC 2021

Phenotypic and Functional Changes in Peripheral Blood Natural Killer Cells in Crohn Disease Patients.

Mediators of inflammation

Samarani S,Sagala P,Jantchou P,Grimard G,Faure C,Deslandres C,Amre DK,Ahmad A

Published figure using CD3 monoclonal antibody (Product # 17-0036-42) in Flow Cytometry

Fri Dec 11 00:00:00 UTC 2020

HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

Journal of Cancer

Kim YD,Park SM,Ha HC,Lee AR,Won H,Cha H,Cho S,Cho JM

Published figure using CD3 monoclonal antibody (Product # 17-0036-42) in Flow Cytometry

Mon Sep 28 00:00:00 UTC 2020

Synthetic Abortive HIV-1 RNAs Induce Potent Antiviral Immunity.

Frontiers in immunology

Stunnenberg M,Sprokholt JK,van Hamme JL,Kaptein TM,Zijlstra-Willems EM,Gringhuis SI,Geijtenbeek TBH

Published figure using CD3 monoclonal antibody (Product # 17-0036-42) in Flow Cytometry

Fri Feb 19 00:00:00 UTC 2021

Infection of human lymphomononuclear cells by SARS-CoV-2.

bioRxiv : the preprint server for biology

Pontelli MC,Castro IA,Martins RB,Veras FP,Serra L,Nascimento DC,Cardoso RS,Rosales R,Lima TM,Souza JP,Caetité DB,de Lima MHF,Kawahisa JT,Giannini MC,Bonjorno LP,Lopes MIF,Batah SS,Siyuan L,Assad RL,Almeida SCL,Oliveira FR,Benatti MN,Pontes LLF,Santana RC,Vilar FC,Martins MA,Cunha TM,Calado RT,Alves-Filho JC,Zamboni DS,Fabro A,Louzada-Junior P,Oliveira RDR,Cunha FQ,Arruda E

17-0036-42 was used in Flow Cytometry to show that SARS-CoV-2 infection of blood circulating leukocytes in COVID-19 patients may have important implications for disease pathogenesis, immune dysfunction, and virus spread within the host.

Fri Aug 07 00:00:00 UTC 2020

Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors.

Molecules (Basel, Switzerland)

Choi JW,Lim S,Kang JH,Hwang SH,Hwang KC,Kim SW,Lee S

17-0036 was used in Flow cytometry/Cell sorting to evaluate the anticancer potential of isolated extracellular vesicles from expanded and activated NK-cell-enriched lymphocytes.

Mon Nov 09 00:00:00 UTC 2020

Cytotoxic effects of ex vivo-expanded natural killer cell-enriched lymphocytes (MYJ1633) against liver cancer.

BMC cancer

Choi JW,Lee ES,Kim SY,Park SI,Oh S,Kang JH,Ryu HA,Lee S

Published figure using CD3 monoclonal antibody (Product # 17-0036-42) in Flow Cytometry

Mon Aug 19 00:00:00 UTC 2019

The Obese Liver Environment Mediates Conversion of NK Cells to a Less Cytotoxic ILC1-Like Phenotype.

Frontiers in immunology

Cuff AO,Sillito F,Dertschnig S,Hall A,Luong TV,Chakraverty R,Male V

Published figure using CD3 monoclonal antibody (Product # 17-0036-42) in Flow Cytometry

Wed Oct 21 00:00:00 UTC 2020

Effect of TGF-β1 on blood CD4⁺CD25^high regulatory T cell proliferation and Foxp3 expression during non-small cell lung cancer blood metastasis.

Experimental and therapeutic medicine

Hu Y,Qi W,Sun L,Zhou H,Zhou B,Yang Z

17-0036-42 was used in Flow cytometry/Cell sorting to provide a theoretical basis for the immunomodulatory processes during non-small cell lung cancer (NSCLC) blood metastasis.

Wed Aug 01 00:00:00 UTC 2018

Psoriasis patients demonstrate HLA-Cw*06:02 allele dosage-dependent T cell proliferation when treated with hair follicle-derived keratin 17 protein.

Scientific reports

Yunusbaeva M,Valiev R,Bilalov F,Sultanova Z,Sharipova L,Yunusbayev B

Published figure using CD3 monoclonal antibody (Product # 17-0036-42) in Flow Cytometry

Tue Apr 17 00:00:00 UTC 2018

Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.

Frontiers in pharmacology

Guo X,Jiang H,Shi B,Zhou M,Zhang H,Shi Z,Du G,Luo H,Wu X,Wang Y,Sun R,Li Z

Published figure using CD3 monoclonal antibody (Product # 17-0036-42) in Flow Cytometry

Thu Sep 28 00:00:00 UTC 2023

Elevated oxytocin and noradrenaline indicate higher stress levels in allergic rhinitis patients: Implications for the skin prick diagnosis in a pilot study.

PloS one

Gotovina J,Pranger CL,Jensen AN,Wagner S,Kothgassner OD,Mothes-Luksch N,Palme R,Larenas-Linnemann D,Singh J,Mösges R,Felnhofer A,Glenk LM,Jensen-Jarolim E

Mon Aug 13 00:00:00 UTC 2018

An In Vitro Potency Assay for Monitoring the Immunomodulatory Potential of Stromal Cell-Derived Extracellular Vesicles.

International journal of molecular sciences

Pachler K,Ketterl N,Desgeorges A,Dunai ZA,Laner-Plamberger S,Streif D,Strunk D,Rohde E,Gimona M

17-0036 was used in Flow cytometry/Cell sorting to support the application of a cell-based in vitro potency assay for reproducibly determining the immunomodulatory potential of extracellular vesicles.

Sat Jul 01 00:00:00 UTC 2017

The major cow milk allergen Bos d 5 manipulates T-helper cells depending on its load with siderophore-bound iron.

PloS one

Roth-Walter F,Pacios LF,Gomez-Casado C,Hofstetter G,Roth GA,Singer J,Diaz-Perales A,Jensen-Jarolim E

17-0036 was used in Flow cytometry/Cell sorting to investigate the ability of Bos d 5 to bind to iron-siderophore complexes and tested its immune-modulatory properties.

Tue Feb 02 00:00:00 UTC 2016

Biallelic loss-of-function mutation in NIK causes a primary immunodeficiency with multifaceted aberrant lymphoid immunity.

Nature communications

Willmann KL,Klaver S,Doğu F,Santos-Valente E,Garncarz W,Bilic I,Mace E,Salzer E,Conde CD,Sic H,Májek P,Banerjee PP,Vladimer GI,Haskoloğlu S,Bolkent MG,Küpesiz A,Condino-Neto A,Colinge J,Superti-Furga G,Pickl WF,van Zelm MC,Eibel H,Orange JS,Ikincioğulları A,Boztuğ K

17-0036 was used in Flow cytometry/Cell sorting to study the effect of a biallelic mutation in the gene encoding NF-κB-inducing kinase on immune cell populations.

Wed Nov 19 00:00:00 UTC 2014

CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.

PloS one

Zhang W,Nie L,Wang Y,Wang XP,Zhao H,Dongol S,Maharjan S,Cheng L

Published figure using CD3 monoclonal antibody (Product # 17-0036-42) in Flow Cytometry

Fri Oct 13 00:00:00 UTC 2017

A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Kap YS,van Meurs M,van Driel N,Koopman G,Melief MJ,Brok HP,Laman JD,'t Hart BA

Tue Dec 01 00:00:00 UTC 2009

生物信息学

蛋白别名: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

查看更多收起

基因别名: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ