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产品信息
RP-103744
产品规格
种属
Human
Expression System
E. coli
氨基酸序列
RTFGDVLQPAKPEYRVGEVAEVIFVGANPKNSVQNQNHQT
标签
His-ABP-tag
分类
Recombinant
类型
Protein
纯度
>80% by SDS-PAGE and Coomassie blue staining
偶联物
形式
Liquid
浓度
≥5.0 mg/mL
纯化类型
purified
保存液
1M urea/PBS, pH 7.4
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
产品详细信息
Highest antigen sequence indentity to the following orthologs: Mouse (85%), Rat (85%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-63928. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
靶标信息
Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5-8.5. ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed primarily expressed with higher levels in the intestine, kidney, skeletal muscle and heart. Recent studies indicate that ASAH2 encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract.
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生物信息学
蛋白别名: Acylsphingosine deacylase 2; BCDase; hCD; LCDase; mitochondrial ceramidase; N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; N-acylsphingosine amidohydrolase 2; N-CDase; Neutral ceramidase; neutral/alkaline ceramidase; Non-lysosomal ceramidase
基因别名: ASAH2; BCDase; HNAC1; LCDase; N-CDase; NCDase
UniProt ID: (Human) Q9NR71
Entrez Gene ID: (Human) 56624
