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产品信息
RP-020
产品规格
种属
Human
已发表种属
Not Applicable
Expression System
E. coli
氨基酸序列
Full-length
分子量
18 kDa
分类
Recombinant
类型
Protein
纯度
>95% by SDS-PAGE
内毒素浓度
<0.1 EU/µg
活性
Catalyzes the cis-trans-isomeration of X-Pro-peptide bonds. The protein folding that is limited by the isomeration of X-Pro-peptide bonds can be accelerated by cyclophilin A. Also binds to cyclosporins and the cyclophilin A/cyclosporin A.
偶联物
形式
Liquid
浓度
0.8 mg/mL
纯化类型
purified
保存液
20mM tris HCl, pH 7.8
内含物
no preservative
保存条件
4° C
运输条件
Wet ice
产品详细信息
RP-020 can be used to catalyze the cis-trans-isomerization of X-Pro-peptide bonds. Protein folding reactions that are limited by the isomerization of X-Pro-peptide bonds are accelerated by cyclophilin A.
RP-020 is a full-length human recombinant Cyclophilin A protein expressed in E. coli. Freezing of the enzyme or storage of the enzyme in diluted form could lead to loss of activity.
Caution: Loss of protein may occur at high dilutions due to adsorption to container surfaces. Freezing of the enzyme or storage of the enzyme in diluted form could lead to loss of activity. In order to best maintain the endotoxin specification and peptidyl prolyl isomerase activity, it is recommended that aliquots be withdrawn with sterile pipet tips, if possible under a sterile hood.
靶标信息
Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: cyclophilin; Cyclophilin A; Cyclosporin A-binding protein; CyP A; EC 5.2.1.8; epididymis secretory sperm binding protein Li 69p; HGNC:9253; MGC117158; MGC12404; MGC23397; Peptidyl-prolyl cis-trans isomerase A; Peptidyl-prolyl cis-trans isomerase A, N-terminally processed; peptidylprolyl isomerase A (cyclophilin A); PPIase; PPIase A; rotamase; Rotamase A; T cell cyclophilin
基因别名: CYPA; CYPH; HEL-S-69p; PPIA
UniProt ID: (Human) P62937
Entrez Gene ID: (Human) 5478
