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产品信息
PHG6045
应用
建议稀释比
已发表文章
产品规格
种属
Human
Expression System
E. coli
氨基酸序列
NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
分子量
6.2 kDa
分类
Recombinant
类型
Protein
纯度
>98% by SDS-PAGE and HPLC
内毒素浓度
<1 EU/µg
活性
ED50 ≤ 0.1 ng/mL; determined by the dose-dependent proliferation of Balb/c 3T3 cells.
偶联物
形式
Lyophilized
纯化类型
purified
内含物
no preservative
保存条件
-20°C
运输条件
Wet ice
产品详细信息
Carrier-Free
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute EGF in sterile, distilled water to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in aqueous buffers supplemented with carrier protein.
Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles.
靶标信息
EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.
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生物信息学
蛋白别名: beta-urogastrone; EGF; Epidermal growth factor; H-EGF; Pro-epidermal growth factor
基因别名: EGF; HOMG4; URG
UniProt ID: (Human) P01133
Entrez Gene ID: (Human) 1950
