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产品信息
RIL2I
应用
建议稀释比
已发表文章
产品规格
种属
Human
已发表种属
Not Applicable
Expression System
P. pastoris
氨基酸序列
Mouse IL-2, amino acids 21-153 (Accession # NP_000577)
分子量
15 kDa
分类
Recombinant
类型
Protein
纯度
>95% by SDS-PAGE
活性
ED50 = 13.5- 20.3 ng/mL; determined by measuring the proliferation of mouse HEK-Blue cells.
偶联物
形式
Lyophilized
纯化类型
purified
保存液
PBS
内含物
no preservative
保存条件
4° C
运输条件
Wet ice
产品详细信息
Mature human IL-2 was expressed in Pichia pastoris, X-33. GenBank Accession # NP_000577, amino acids 21 - 153. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: APTSSSTKKT. Purity is >95% by SDS-PAGE analyses.
Activity: The biological activity was determined by measuring the proliferation of mouse HEK-Blue cells.
Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.
Reconstitution and Dilution: Store lyophilized material at 4ºC. Reconstitute in 1 mL of distilled or deionized water. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70ºC, avoiding multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.
靶标信息
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: aldesleukin; H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; M-IL-2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF
基因别名: IL-2; IL2; lymphokine; TCGF
UniProt ID: (Human) P60568
Entrez Gene ID: (Human) 3558
