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产品信息
PHG0041
应用
建议稀释比
已发表文章
产品规格
种属
Human
Expression System
E. coli
氨基酸序列
SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT
分子量
25.4 kDa
分类
Recombinant
类型
Protein
纯度
>95% by SDS-PAGE
内毒素浓度
<0.1 ng/µg
活性
ED50 ≤ 5 ng/mL; determined by the dose-dependent proliferation of BALB/c 3T3 cells.
偶联物
形式
Lyophilized
纯化类型
purified
内含物
no preservative
保存条件
4° C
运输条件
Wet ice
产品详细信息
Carrier-Free
Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in 100mM acetic acid containing 0.1% BSA to 0.1-1.0 mg/mL to regain full activity. Apportion the reconstituted protein into working aliquots and store at ≤-20°C. Make any further dilutions of the reconstituted protein in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA.
Storage: Store the lyophilized protein at 2-8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.
靶标信息
The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: becaplermin; FLJ12858; H-PDGF-BB; PDGF subunit B; PDGF, B chain; PDGF-2; platelet-derived growth factor 2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); Platelet-derived growth factor subunit B; platelet-derived growth factor, beta polypeptide (oncogene SIS); Proto-oncogene c-Sis
基因别名: c-sis; IBGC5; PDGF-2; PDGF2; PDGFB; SIS; SSV
UniProt ID: (Human) P01127
Entrez Gene ID: (Human) 5155
