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产品信息
150-14T-25UG
产品规格
种属
Human
已发表种属
Human
Expression System
E. coli
氨基酸序列
ARFALTVVRH GETRFNKEKI IQGQGVDEPL SETGFKQAAA AGIFLNNVKF THAFSSDLMR TKQTMHGILE RSKFCKDMTV KYDSRLRERK YGVVEGKALS ELRAMAKAAR EECPVFTPPG GETLDQVKMR GIDFFEFLCQ LILKEADQKE QFSQGSPSNC LETSLAEIFP LGKNHSSKVN SDSGIPGLAA SVLVVSHGAY MRSLFDYFLT DLKCSLPATL SRSELMSVTP NTGMSLFIIN FEEGREVKPT VQCICMNLQD HLNGLTETRG GGYGRKKRRQ RRR
标签
TAT-fusion
分子量
31.6 kDa
分类
Recombinant
类型
Protein
纯度
≥ 95% by SDS-PAGE gel and HPLC analyses.
内毒素浓度
<1 EU/µg
活性
Pretreatment with TIGAR-TAT for 4 hrs, using a concentration range 0.1-5.0 ug/ml, protects U2OS cells from apoptosis induced by hydrogen peroxide.
偶联物
形式
Lyophilized
纯化类型
purified
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
产品详细信息
Recombinant Human TIGAR-TAT expressed in E.coli is a 31.6 kDa protein containing 283 amino acid residues, including the 269 residues of full-length TIGAR fused to a 14-residue C-terminal peptide containing the TAT transduction domain (GGGYGRKKRRQRRR).
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis
靶标信息
TIGAR is a p53-inducible enzyme that catalyzes the hydrolysis of fructose-2-6 bisphosphate (F-2-6-BP) to fructose-6-phosphate and inorganic phosphate. F-2-6-BP is a powerful activator of 6-phosphofructose-1 kinase, the rate limiting enzyme of glycolysis. By lowering the intracellular level of F-2-6-BP, TIGAR expression leads to increased glucose processing via the pentose phosphate pathway, the major cellular source for NADPH. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other intracellular proteins into primary, as well as transformed, cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Fructose-2,6-bisphosphatase TIGAR; fructose-2,6-bisphosphate 2-phosphatase; probable fructose-2,6-bisphosphatase TIGAR; TIGAR; TP53-induced glycolysis and apoptosis regulator; TP53-induced glycolysis regulatory phosphatase; transactivated by NS3TP2 protein
基因别名: C12orf5; FR2BP; TIGAR
UniProt ID: (Human) Q9NQ88
Entrez Gene ID: (Human) 57103
