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产品信息
RP-91795
产品规格
种属
Human
Expression System
E. coli
氨基酸序列
DCPHVDDCVFAAEFMSNDYVRVTQLYCDGVGVQYKDYIQSERNLEFDICSIWCSKPISVLQDYCDAIKINIFWPLLFQHQNSSVISRLHPCVDANNSRASEINLKKLQHLELMEDIVDLAKKVANDS
标签
His-ABP-tag
分类
Recombinant
类型
Protein
纯度
>80% by SDS-PAGE and Coomassie blue staining
偶联物
形式
Liquid
浓度
≥5.0 mg/mL
纯化类型
purified
保存液
PBS/1M urea, pH 7.4
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
产品详细信息
Highest antigen sequence indentity to the following orthologs: Mouse (77%), Rat (77%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-54942. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
靶标信息
E3 ubiquitin-protein ligase which catalyzes the formation of 'Lys-48'-polyubiquitin chains (PubMed:20059950, PubMed:30696809). Mediates the ubiquitination and subsequent degradation of phosphorylated Akt (AKT1, AKT2 and AKT3) in the nucleus (PubMed:20059950). Acts as a terminal regulator of Akt signaling after activation; its phosphorylation by Akt, which is a prerequisite for ubiquitin ligase activity, suggests the existence of a regulation mechanism required to control Akt levels after activation (PubMed:20059950). Positively regulates TGFB1-induced epithelial-mesenchymal transition and myofibroblast differentiation by mediating the ubiquitination and subsequent degradation of SMURF2 (PubMed:30696809). Regulates neuronal differentiation by regulating actin remodeling and Golgi organization via a signaling cascade involving RHOA, CIT and ROCK (PubMed:17488780, PubMed:24695496). Inhibits cell proliferation (PubMed:30203323). [UniProt]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: E3 ubiquitin-protein ligase TTC3; Protein DCRR1; RING finger protein 105; RING-type E3 ubiquitin transferase TTC3; Tetratricopeptide repeat protein 3; TPR repeat protein 3; TPR repeat protein D
基因别名: DCRR1; RNF105; TPRD; TPRDIII; TTC3
UniProt ID: (Human) P53804
Entrez Gene ID: (Human) 7267
