Gibco

Human Vitronectin, Animal-Free Recombinant Protein, PeproTech®

产品信息

AF-140-09-500UG

应用

建议稀释比

已发表文章

功能检测 (Functional)

Assay-dependent

体外检测 (IV)

查看5篇已发表文章 5篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属

Human

已发表种属

Human

Expression System

HEK293 cells

氨基酸序列

DQESCKGRCT EGFNVDKKCQ CDELCSYYQS CCTDYTAECK PQVTRGDVFT MPEDEYTVYD DGEEKNNATV HEQVGGPSLT SDLQAQSKGN PEQTPVLKPE EEAPAPEVGA SKPEGIDSRP ETLHPGRPQP PAEEELCSGK PFDAFTDLKN GSLFAFRGQY CYELDEKAVR PGYPKLIRDV WGIEGPIDAA FTRINCQGKT YLFKGSQYWR FEDGVLDPDY PRNISDGFDG IPDNVDAALA LPAHSYSGRE RVYFFKGKQY WEYQFQHQPS QEECEGSSLS AVFEHFAMMQ RDSWEDIFEL LFWGRTSAGT RQPQFISRDW HGVPGQVDAA MAGRIYISGM APRPSLAKKQ RFRHRNRKGY RSQRGHSRGR NQNSRRPSRA TWLSLFSSEE SNLGANNYDD YRMDWLVPAT CEPIQSVFFF SGDKYYRVNL RTRRVDTVDP PYPRSIAQYW LGCPAPGHL

分子量

52.2 kDa

分类

Recombinant

类型

Protein

纯度

≥ 95% by SDS-PAGE gel and HPLC analyses.

内毒素浓度

<0.1 EU/µg

活性

Recombinant human Vitronectin promotes attachment of hESC and iPSC in serum-free, feeder conditions at 5ug/ml.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

纯化类型

purified

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

产品详细信息

Recombinant Human Vitronectin is a 459 amino acid, single-chain, monomeric protein, which migrates at an apparent molecular weight of 75 kDa by SDS-PAGE under reducing conditions. The calculated molecular weight of Recombinant Human Vitronectin is 52.2 kDa.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

靶标信息

VTN is a member of the pexin family. This protein is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. The protein is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Accelerated differentiation of human induced pluripotent stem cells into regionally specific dorsal and ventral spinal neural progenitor cells for application in spinal cord therapeutics.

Frontiers in neuroscience

Huntemer-Silveira A,Malone D,Frie A,Walsh P,Parr AM

AF-140-09 was used in Cell Culture to describe here the production of a diverse population of both ventral spinal and dorsal horn progenitor cells.

Sat Feb 10 00:00:00 UTC 2024

The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury.

Neurobiology of disease

Triplet EM,Kim HN,Yoon H,Radulovic M,Kleppe L,Simon WL,Choi CI,Walsh PJ,Dutton JR,Scarisbrick IA

AF-140-09 was used in In vitro experiments to identify the thrombin receptor (Protease Activated Receptor 1 (PAR1)) as an integral facet of this machine with roles in regulating neurite growth through a growth factor- and cholesterol-dependent mechanism.

Sat May 01 00:00:00 UTC 2021

Automating Human Induced Pluripotent Stem Cell Culture and Differentiation of iPSC-Derived Retinal Pigment Epithelium for Personalized Drug Testing.

SLAS technology

Truong V,Viken K,Geng Z,Barkan S,Johnson B,Ebeling MC,Montezuma SR,Ferrington DA,Dutton JR

AF-140-09 was used in Cell Culture to demonstrate scalable, reproducible culture and differentiation of hiPSC lines from individuals on the TECAN Fluent platform and illustrate the potential for end-to-end automation of hiPSC-based personalized drug testing.