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产品信息
RP-101284
产品规格
种属
Human
Expression System
E. coli
氨基酸序列
SLRQHQGDYDGQMLFSCGDEGKAFLDTFTLLDSQMTHAEVRPFRCLPCGNVFKEKS
标签
His-ABP-tag
分类
Recombinant
类型
Protein
纯度
>80% by SDS-PAGE and Coomassie blue staining
偶联物
形式
Liquid
浓度
≥5.0 mg/mL
纯化类型
purified
保存液
1M urea/PBS, pH 7.4
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
产品详细信息
Highest antigen sequence indentity to the following orthologs: Mouse (43%), Rat (43%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-62207. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
靶标信息
ZNF304 acts as transcriptional regulator and plays a role in gene silencing. It probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of several tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. ZNF304 is also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) by inducing trimethylation of Lysine-27 of histone H3 (H3K27me3) in a Polycomb group (PcG) complexes-dependent manner. The protein associates at promoter regions of TSGs and mediates the recruitment of the corepressor complex containing the scaffolding protein TRIM28, methyltransferase DNMT1 and histone methyltransferase SETDB1 and/or the PcG complexes at those sites. It acts as a transcription factor involved in the metastatic cascade process by inducing cell migration and proliferation and gain resistance to anoikis of ovarian carcinoma (OC) cells via integrin-mediated signaling pathways. It associates with the ITGB1 promoter and positively regulates beta-1 integrin transcription expression. It promotes angiogenesis and tumor growth.
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生物信息学
蛋白别名: KRAB-containing zinc finger protein; Zinc finger protein 304
基因别名: ZNF304
UniProt ID: (Human) Q9HCX3
Entrez Gene ID: (Human) 57343
