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产品信息
AF-315-09-500UG
产品规格
种属
Mouse
已发表种属
Human,
Mouse
Expression System
E. coli
氨基酸序列
NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR
分子量
6 kDa
分类
Recombinant
类型
Protein
纯度
≥ 98% by SDS-PAGE gel and HPLC analyses.
内毒素浓度
<0.1 EU/µg
活性
The ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10^7 units/mg.
偶联物
形式
Lyophilized
纯化类型
purified
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
产品详细信息
Recombinant Murine EGF is a 6.0 kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide bonds.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis
靶标信息
EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: EGF; Epidermal growth factor; H-EGF; Pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF)
基因别名: AI790464; Egf
UniProt ID: (Mouse) P01132
Entrez Gene ID: (Mouse) 13645
