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产品信息
PEP-183
产品规格
种属反应
Mouse
分类
Synthetic
类型
Peptide
偶联物
形式
Lyophilized
浓度
0.5 mg/mL
纯化类型
purified
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
产品详细信息
PEP-183 is a 17 amino acid synthetic peptide whose sequence is derived from mouse PDE6 beta protein. The sequence of this peptide is (amino to carboxy terminus): Synthetic Peptide: H (20)-Q-Y-F-G- (K/R)-K-L-S-P-E-N-V-A-G-A-C (36)
This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and mouse PDE6 beta, catalog # PA1-722. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (~70-fold) for competitive inhibition of antibody-protein binding reactions.
Reconstitute with 0.1 mL of distilled water.
靶标信息
The second messengers cAMP and cGMP are key regulatory molecules that are involved in a wide variety of signal transduction pathways. Levels of cAMP and cGMP are regulated by their rate of synthesis by nucleotide cyclases and by their rate of hydrolysis by cyclic nucleotide phosphodiesterases (PDEs). PDEs form a superfamily of enzymes that catalyze the conversion of 3-prime, 5-prime-cyclic nucleotides to the corresponding nucleoside 5-prime-monophosphates. PDE6 is the effector enzyme in the G protein-mediated signal transduction cascade in the visual system. There are five different subunits consisting of rod and cone specific catalytic subunits: alpha' (Cone), alpha (Rod), and beta (Rod), the inhibitory subunit gamma, and subunit delta of unknown function (which likely interacts with many other proteins besides the PDE6 family). The catalytic core of the PDE6 system is comprised of alpha'/alpha' homodimers in the cone and alpha/beta heterodimers in the rod. The C-terminus of both the catalytic and inhibitory subunits is modified by methylation, myristyolation and prenylation which have been shown to be critical for proper complex assembly and membrane association.
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生物信息学
蛋白别名: cGMP-phosphodiesterase beta-subunit; GMP-PDE beta; phosphodiesterase, cGMP, rod receptor, beta polypeptide; retinal degeneration 1; Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta
基因别名: Mpb; Pde6b; Pdeb; r; rd; rd-1; rd1; rd10
UniProt ID: (Mouse) P23440
Entrez Gene ID: (Mouse) 18587
